Isolation And Identification Of Taxol-producing Endophytic Fungis From Taxus Chinensis

Endophytic fungi isolates (237) were isolated from Taxus chinensis grown in Qinba Mountains of China by tissue culture. Among these endophytic fungi, a total of 120, 35 and 82 isolates were respectively from the host plants in Liuba, Nazheng and Feping.The submerged cultures of the endophytic strains in the liquid medium were subjected to ethyl acetate extract, and then respectively screened with UV, HPLC, and HPLC-MS. The results showed that 9 isolates could produce taxol, of which 7 stains were from Feping, and 2 stains were from Liuba. Among these stains, LB-10 produced taxol with the highest yield of 846.1μg / L. The species and numbers of these endophytic taxol-producing fungi are different, which may be related to the host plants grown in different geographical environments.Taxol-producing endophytic fungi were classified into 9 genera, based on traditional taxonomic and molecular biology methods. The results were as follows: Tax-4 was grouped into Fusarium solani, and Tax-12, Tax-13, Tax-19, LB-10, Tax-38, G23, Tax-21 and G8 was Phomopsis sp., Aspergillus sp., Xylaria sp., Metarhizium anisopliae, Ceratobasidium sp., Alternaria sp., and Cladosporium sp., respectively. Deuteromycotina was the dominant group, counting 75% of these taxol-producing fungi. The endophytic fungus, LB-10, may be a potential production source of fungal taxol.