Genegenetic Polymorphism Research On Tibetan Pig MHC-DQB1

Porcine major histocompatibility complex (Major Histocompatibility Complex, referred to as MHC), also called SLA (Swine Lymphocyte Antigen), or swine leukocyte antigen. SLA and pig production, reproductive traits and disease resistance and other related properties, as a marker assisted selection in pig genetics and breeding applications are more and more attention. SLA in the pig chromosome 7. By the MHC classâ… pig,â…¢andâ…¡3 gene cluster or regional composition, SLAâ… genes and SLAâ…¢genes located on the short arm of chromosome 7, SLAâ…¡genes in chromosome long arm. Classâ…¡genes includes DRA, DRB, DQA, DQB, DOB, DPA, TAP, and LMP and other sub-regions.This study from the Tibet pig MHC-DQB1 gene polymorphism departure for possession of pig MHC-DQB1 genetic polymorphism and to provide theoretical basis and technical reserve.According to GenBank pig SLA-DQB1 gene sequence (accession number NC₀10449.1) designed eight pairs of primers, a total of 90 pigs of the three species were sequenced, to find SNPs. In P1, P2, P3, P8 primers were found in 13 SNPs. Intron4-1169 possession of pig sequences from the T→G mutation; Intron4-1159 possession of pig sequences from the G→C mutation; Intron4-1146 possession of pig sequences from the C→G mutation; Intron4-1084 possession of the pig sequence A→C mutation; Intron4-1000 possession of pig sequences from the C→G mutation; Intron4-999 possession of pig sequences from the A→G mutation. Intron3-417 possession of pig sequences from the G→T mutation. Intron2-3348 possession of pig sequences from the G→A mutation; Intron2-3379 possession of pig sequences from the C→G mutation. Exon1-104 bits long Deficiency of Yorkshire and Duroc, possession of a pig into the phenomenon. Intron1-300 possession of pig sequences from the G→A mutation. Intron1-329, Intron1-369 possession of pig sequences from the G→A mutation. The web site:http://www.cbrc.jp/research/db/TFSEARCH.html the TFSEARCH (ver 1.3) software before and after the 13 mutations were predicted transcription factors, Intron4-1169, Intron4-1169 bases in the T→G, G→C mutation had reduced to a predictable regulatory element binding sites for the transcription factor AML-1a (high prediction score 85.4). Intron4-1146 occurs at base position C→G mutation can be predicted after the addition of two regulatory elements to the binding sites, respectively, transcription factor MZF1 (higher prediction score 88.7) and the transcription factor NF-kap (higher prediction score 85.2). Intron4-1084 occurred nucleotide A→C mutation, the two can be predicted to reduce the regulatory element binding sites, respectively, the transcription factor GATA-1 (high prediction score 89.0) and the transcription factor GATA-2 (higher prediction score 85.8). Intron4-1000, Intron4-999 occurs by nucleotide C→G, the A→G mutation, the addition of a regulatory element can predict the binding sites for transcription factors MZF1 (higher prediction score 94.8), reduced to a predictable regulatory element binding sites for transcription factor Sp1 (higher prediction score 87.7). Intron3-417 occurs at base position of the G→T mutation and the second intron of 3348 base took place in the G→A mutation does not lead to any potential regulatory elements and protein binding sites change. Intron2-3379 took place from the nucleotide C→G mutation, reduced to a predictable regulatory element binding sites for the transcription factor CDP CR (higher prediction score 87.7). Exon1-104 bits long into the phenomenon of Landrace and Duroc, Tibet pigs Deficiency. Intron1-300 occurs by the nucleotide G→A mutation, the addition of a regulatory element can predict the binding sites for transcription factors STATx (higher prediction score 86.5), reduced to a predictable regulatory elements binding sites for transcription factor IK-2 (high prediction score 86). Intron1-329 G→A mutation, the addition of a regulatory element can predict the binding sites for transcription factors STATx (higher prediction score 86.5), reduced to a predictable regulatory element binding sites for transcription factor IK-2 (high prediction score 86). Intron1-369 occurs by the nucleotide G→A mutation, increased regulation of the three components can be predicted binding sites for transcription factor IK-2 (high prediction score 88.6), the transcription factor C / EBPb ( higher prediction score 88.1), transcription factor Lyf-1 (high prediction score 85.7); reduced to a predictable regulatory element binding sites for transcription factors MZF1 (higher prediction score 91.3).With restriction enzymes Dde I analysis of SLA-DQB1 Intron3-417 mutation at base position, possession of the body appears pigs AA, AB and BB 3 genotypes, of which AB genotype was the highest 56.00%; BB genotype lowest 18%; allele frequency of A and B were 54% and 46%. Chi-square analysis showed that possession of pigs in vivo SLA-DQB1 Intron3-417 nucleotide Dde I restriction enzyme genotype distribution consistent with Hardy-Weinberg equilibrium.Landrace and Duroc only BB genotype.The present study found that the possession of the pig MHC-DQB1 genetic polymorphism for the possession of pig MHC-DQB1 genetic polymorphism study provides some basis, while Tibetan pig is a good disease-resistant varieties MHC can be used as genetic markers in animal disease resistance, disease resistance selection in the secondary role. Tibetan pig MHC-DQB1 mutation on disease resistance is not the decisive factor will be further study.