Comparative Analysis Of Soil Microbial Flora Of Diffrerent Forests In Tonglu And Tiantai Of Zhejiang Province

Soil microbe as an important component in forest ecosystem,which was used as an indicator to evaluate forest health, plays an important role in energy flow of soil and progress of material cycle. Therefore, it was significant to investigate the effects of floristic changes in soil microbe of forest on the healthy development of forest ecosystems.In the present study, three woodlands were chosen from Tonglu, Zhejiang Province: Broadleaf forest (sample A), Masson pine-broadleaved mixed forest (sample B), and Masson pine plantation (sample C), and two woodlands were chosen from Tiantai:Masson pine forest (sample D) and Broadleaf forest (sample E), all of which were performed microbiotic comparative analysis. Microflora in 0～50 cm soil layers of aboved 5 woodlands was observed using morphological analysis combined with molecular biology identification. The main results were as followed:1. The results showed that the quantitative proportions of microorganism in 5 forests communities were:Bacteria was the main microbial group, followed by actinomycetes, and eumycete was smallest.2. There were significant differences with a vertical distribution in the quantity of soil microbe, the surface layer of which was significant higher compared to bottom layer, and all the vertical distributions of bacteria, fungi and actinomycetes had a gradually decreasing tendency with the increase of the soil depth.3. There were significant differences of the quantity of soil microbe and distribution of dominant species in different forests and different seasons suggesting that the season, the differences of forest vegetation and edaphic condition were the principal factors to affect the quantity of soil microbe.(1) The comparison of bacteria flora in 5 forests:The quantities of bacteria in 5 forests:There was three significant differences bacteria quantities between sample A and B,C in the surface layer, while no significant difference between sample D and E. There was significant differences bacteria quantities between sample B and A,C, and between sample D and E in the bottom layer, while no significant difference between sample A and C.The quantities of bacteria in the four seasons:in sample A and B germ had the maximum number in winter in two soil horizons. The surface layer of sample C and D, germ had the maximum number in winter and in the bottomsoil layer, the number reached its maximum in fall. In sample E surface layer, the peak of germ number appearred in spring and in bottom layer, it appearred in summer.The dominant species of soil bacteria were Lysinibacillus, Staphylococcus, Burkholderia, Arthrobacter, Paenibacillus, Micrococcus, Pseudomonas and Bacillus.(2) The comparison of fungi flora in 5 forests:The quantities of fungi in 5 forests:There was three significant differences bacteria quantities between sample A and B,C in the surface layer, while significant difference between sample D and E. There was significant differences fungi quantities between sample A and B,C in the bottom layer, while no significant difference between sample A and C, and between sample D and E.The quantities of fungi in the four seasons:in sample A, fungus'number had the maximum number in summer in two soil horizons, and fungus'number had the maximum number in fall in two soil horizons of sample B. The surface layer of sample C, fungus'number had the maximum number in summer and in the bottom soil layer, the number reached its maximum in fall. In sample D, the number reached its maximum in summer in two soil horizons. And in sample E, the fungus'number of two soil horizons reached its maximum in summer.The dominant species of soil fungi included Mortierella, Aspergillus,Penicillium, Trichoderma and Cladosporium.(3) The comparison of actinomycete flora in 5 forests:The quantities of actinomycete in 5 forests:There was significant differences bacteria quantities between sample A and B,C, and between sample D and E in the surface layer, while no significant difference between sample B and C. There was three significant differences actinomycete quantities between sample A and B,C, and between sample D and E. in the bottom layer.The quantities of actinomycete in the four seasons:in sample A, actinomycetes'number had the maximum number in summer in surface layer, while actinomycetes' number of bottom layer had the maximum number in winter. Actinomycetes'number of sample B,it had the maximum number in winter, while the number reached its maximum in spring in bottom layer. The surface layer of sample C, Actinomycetes' number had the maximum number in summer and in the bottom soil layer, the number reached its maximum in fall. In sample D, the number reached its maximum in winter. As for bottom layer, it was in sunmmer. And in sample E, the actinomycetes" number of two soil horizons reached its maximum in summer.The dominant species of soil actinomycete were Streptomyces, Nocardia,Micromonospora and Kitasatospora.