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Studies On Extraction, Identification And Antioxidant Capacity Of Blue Honeysuckle (Lonicera Caerulea L.)

Posted on:2011-01-06Degree:MasterType:Thesis
Country:ChinaCandidate:J ZhaoFull Text:PDF
GTID:2143360308481816Subject:Pomology
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In this paper, the L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica which is the main cultivars of Blue honeysuckle were used as experimental material to research the extraction technology of phenols in its leaves and berries,and analysis the trend of phenols in different periods.Three cultivars including L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica, Blue bird(L.caerulea subsp.Kamtschatica) and Blue spindle (L.caerulea ubsp.Kamtschaticas), and five wild species including (L.caerulea var. edulis),(L.caerulea subsp.pallasii),(L.boczkarnikowii), (L.caerulea subsp.altaicai)and(L.caerulea subsp. emphyllocalyx) as the test material to determine the content of phenols in their leaves and berries,and identify the compone- nts in different speci- es and varieties of Blue honeysuckle,At last,analysis the vitro antioxidant capacity of leaves and berries.During the extraction to phenols in leaf and berries of Blue honeysuckle, the L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica which is the main cultivars of Blue honeysuckle were used as experimental material to screen the optimal conditions using ethanol extraction met- hod. Consider the four influencing factors, including the ethanol concentration, ethanol volume, extracting temperature and extracting time for a single-factor test, and basing on single-factor made orthogonal experimental design to choose the optimum extraction process of phenols in leaves and berries of Blue honeysuckle.Using six methods, including ferric reducing antioxidant power, fraction with anti-lipid per- oxidation, superoxide anion radical scavenging activity, hydroxyl free radical scavenging activity, DPPH radical scavenging activity, ABTS radical scavenging activity, analyze the antioxidant cap- acity of Blue honeysuckle.The study's major findings are as follows:1. Through the single factor and orthogonal tests of the main influencing factors were determed- ined the optimal conditions for phenolic extraction. The best conditions of phenolic extracting from leaves were: ethanol concentration 50 %, ethanol volume 50 mL, extracting temp- erature 50℃, extracting time 2.5 h. In this extracting conditions, the phenolic content in leaves of Blue honeys- honeysuckle were 14.14 mg/g. The best extraction conditions of berries were: ethanol concentration 70, ethanol volum 50 mL, extracting temperature 50℃, extracting time 3.0 h. In this extraction conditions, the phenolic content were 24.44 mg/g. 2. The phenolic contents of leaves in different periods were: the period of no beries 14.13 mg/g, the period of green berries 12.56 mg/g, the period of started coloring 8.99 mg/g, the period of parted coloring 7.64 mg/g, the period of full coloring 7.42 mg/g. The phenlic contents of berri- es in different periods were: the period of green berries 10.34 mg/g, the period of started coloring 11.86 mg/g, the period of parted coloring 15.39 mg/g, the period of full coloring 24.45 mg / g.3. The phenolic contents of leaves in different species and varieties of Blue honeysuckle were: L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica 13.63 mg/g, Blue bird (L.caerulea subsp.Kamtschatica) 9.36 mg/g, Blue spindle (L.caerulea ubsp.Kamtschaticas) 17.10 mg/g, (L.caerulea var. edulis) 12.78 mg/g, (L.caerulea subsp.pallasii) 12.66 mg/g, (L.boczkarnikowii) 13.87 mg/g, (L.caerulea subsp.altaicai) 15.58 mg / g, (L.caerulea subsp. emphyllocalyx) 3.00 mg / g.And the phenolic contents of berries were: L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica 24.19 mg / g, Blue bird (L.caerulea subsp.Kamtschatica) 23.02 mg / g, (L.caerulea subsp.pallasii) 26.33 mg / g, (L.caerulea var. edulis) 24.12 mg / g, (L.caerulea subsp.pallasii) 24.17 mg / g, (L.boczkarnikowii) 25.24 mg / g, (L.caerulea subsp.altaicai) 27.43 mg / g, (L.caerulea subsp. emphyllocalyx) 22.98 mg / g.4. Eight major anthocyanins are identification in berries of Blue honeysuckles, including cyanidin 3, 5-diglucoside, cyanidin 3-glucoside, cyanidin 3-rutinoside, pelargonidin 3-glucoside, pelargonidin 3-glucosid, peonidin 3-glucoside, peonidin 3-rutinoside, cyaniding- (acetoyl)-hexosi- de and cyanidin-derivatives.5. Antioxidant capacity through various analytical methods blue Lonicera antioxidant capacity was evaluated:Ferric reducing ability of the strength of the order: (L.caerulea subsp.altaicai)> (L.caerulea subsp.pallasii)> (L.caerulea subsp.pallasii)> (L.caerulea subsp. emphyllocalyx)>Bluebird>L.cae- rulea subsp.altaicai×L.caerulea subsp.Kamtschatica > Blue spindle> (L.caerulea subsp. Emphyllo- calyx).Anti-lipid peroxidation descending order: (L.caerulea subsp.altaicai) > (L.caerulea subsp.pal- lasii)> (L.caerulea subsp.pallasii)> (L.caerulea subsp. emphyllocalyx)> Bluebird> L.caerulea sub- sp.altaicai×L.caerulea subsp.Kamtschatica > Blue spindle> (L.caerulea subsp. emphyllocalyx).Superoxide anion radicals in descending order: L.caerulea var. edulis)>(L.caerulea subsp.alt- aicai×L.caerulea subsp.Kamtschatica)> Blue spindle > (L.caerulea subsp.pallasii)> (L.caerulea subsp.altaicai)> Bluebird >(L.boczkarnikowii)> (L.caerulea subsp. emphyllocalyx)Scavenging ability in descending order: (L.caerulea subsp.altaicai) > (L.caerulea subsp.pall- asii)> (L.caerulea subsp.pallasii)> (L.caerulea subsp. emphyllocalyx)> Bluebird> L.caerulea subsp. altaicai×L.caerulea subsp.Kamtschatica > Blue spindle> (L.caerulea subsp. emphyllocalyx).DPPH free radical ability to clear the strength of the order: Blue spindle >Bluebird >(L.cae- rulea subsp.pallasii)> (L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica)> (L.boczkami- kowii)>(L.caerulea var. edulis)>(L.caerulea subsp.altaicai)> (L.caerulea subsp. emphyllocalyx).Ability to remove ABTS radical in descending order: Blue spindle > Bluebird >(L.caerulea subsp.pallasii)> (L.caerulea subsp.altaicai×L.caerulea subsp.Kamtschatica)> (L.boczkarmikow- ii)>(L.caerulea var. edulis)>(L.caerulea subsp.altaicai)> (L.caerulea subsp. emphyllocalyx).
Keywords/Search Tags:Blue Honeysuckle, Phenols, Extraction, Antioxidant capacity
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