The Molecular Characterization And Time Course Transcription Of DPV UL10 Gene

This scientific dissertation concentrates on studies on the identified UL10 gene (Genbank accession no. EU195100) from duck plague virus (DPV). The bioinformatics analysis of UL10 gene was analysised.And we have carried out transcriptional analysis. Results are as follows:1. Molecular characterization of DPV UL10 gene The DPV CHv UL10 gene was composed of 1230 base pair,which encoding for a polypeptide of 409 amino acid with an isoelectric point of 9.05. Eight transmembrane helixes were present, which were between aa 21-43,95-117,130-152,163-185,219-241,251-273,280-302 and 322-344. The subcellelar localization preduction showed that the UL10 protein mainly concentrated in endoplasmic reticulum(44.4%). The polypeptide contains 15 possible sites for phosphorylation, which were composed of 8 Serine,4 Threonine and 3 Tyrosine. Sequence comparison of 24 reference herpesvirus strains showed that DPV CHv was more homologous to alphaherpesviruses. The DPV UL10 sequence displayed homologies of 54.5%,53.5%,41.5%, and 46.4%.2. Codon usage bias analysis of DPV UL10 gene Using the CHIPS and CUSP programs, we analysis the ENC, GC content and GC3s content of DPV UL10 gene, which showed that synonymous codon usage bias in the UL10 gene of herpesviruses. phylogenetic tree analysis demonstrated that it has a close evolutionary relationship with alphaherpesviruses. Relevance analysis showed significant relationship between the DPV and yeast UL10 genes (P<0.05).3. Phase analysis of DPV UL10 gene transcription We used the FQ-PCR method to detecte the transcriptional analysis of DPV UL10 gene.The result showed the transcripts rised up at the first 0.5h, then increased quickly and reached a peak at 60h, lowed down slowly but still detected the transcripts of UL10 gene until 72 hours after infection.