Study On The Spationtemporal Expression Of IGFs Genes In Nanjiang Yellow Goats

IGFs played physiological roles on promoting growth, cell proliferation, differentiation and stimulating metabolism. Nanjiang Yellow Goats from 8 different tissues (semimembranosus (BM),triceps brachii muscle (BS), longissimus dorsi muscle (BZ), quadriceps femoris (GST),musculus biceps femoris buttocks (TG), liver, spleen and heart) and 6 different ages (0d, 15d,30d,60d,90d, 120d) were selected to study the spatialtemporal expression of IGFs genes by SYBR Greenâ… fluorescence quantitative PCR technique.The results were as follows:(1) Most of the other tissues didn't have significant difference between male and female goats except a few tissues(P>0.05). It showed that the gender had a little effect on the expression of IGFs genes,but not big.The tissues which had extremely significant differences were mainly liver and spleen.The expression difference of IGFs genes between male and female lambs might be affected by nutrition, hormones and other factors.(2) IGF-â… gene expression quantity of the liver at different periods were the highest, following by the spleen.But for the muscle tissues, the expression quantity of BS was relatively higher at the three time points(0d,15d and 30d). The expression of GST was much higher at 30d,but it had not significant difference between GST and BS (P>0.05). The results indicated that the liver was an important source of protein secretion of IGF-â… .Besides the liver, the other tissues could also secret IGF-â… protein by autocrine or paracrine mechanisms,and IGF-â… gene had an important regulation roles on the spleen development.(3) IGF-â…¡gene expression quantity of the spleen at different.periods were the highest, following by the liver,but the expression of other tissues were relatively lower.It showed that IGF-â…¡gene regulated the development of spleen by paracrine or endocrine possibly.(4) Among eight different tissuses, IGF-â… gene expression quantity at the six different time points showed rising trend,but it was fluctuating rising. Expression quantity of IGF-â… gene reached the highest at 90d. The rising trend from 15d to 90d was relative obviously,but it was declining from 90d to 120d.The result showed that IGF-â… gene could regulate the growth of Nanjiang Yellow Goats.(5) Among eight different tissuses, expression quantity of IGF-â…¡gene at the six different time points also showed rising trend and it had the highest expression at 60d. The expression of IGF-â…¡gene was rising from 15d to 60d,and the rising trend was relative obviously,but it was declining from 60d to 120d. These results showed that the biological functions of IGF-â…¡gene were more concenteated at 60d,and it could regulate the growth and development of Nanjiang Yellow Goats.