Studies On Phenotype And Phsiological Metabolism Of Catharanthus Roseus Under Supplementary UV-B Radiation And Nitrogen Levels

Due to human activities and natural disaster, the increase of UV-B radiation and nitrogen deposition will be the dominant feature of future ecology environment. The increasing of UV-B radiation and nitrogen deposition has significant biological effects to organism. In this thesis, in the artificial climate chamber, the effect of increased UV-B radiation and nitrogen deposition in the ecology environment on Catharanthus roseus's phenotype and physiological metabolism, especially on the metabolism of pharmaceutical active ingredients, such as vindoline, catharanthine and vinblastine, were simulated by adding UV-B lamps and increasing the concentration of nitrate. Based on that we have investigated the physiology and ecology accommodative mechanisms of Catharanthus roseus responding to the increased UV-B radiation and nitrogen deposition when treated time was 3d (short term),7d (middle period) and 15d (long term), respectively. The main results were as follow:1,There were noticeable effects of supplementary UV-B radiation and nitrogen nutrition on the morphology and biomass of Catharanthus roseus, and there were also significant distinction among groups treated with different time period. The plant height, leaf inclined angle, root/shoot and biomass were all markedly reduced under the condition of supplementary UV-B radiation or nitrogen nutrition, or the conditions of both of them together; the leaf number of Catharanthus roseus were raised remarkably under the condition of supplementary UV-B radiation or nitrogen nutrition, however, they have adverse effects on leaf number when adding together.2,There were noticeable oxidation reduction states and antioxidant of Catharanthus roseus under the condition of supplementary UV-B radiation and nitrogen nutrition, and there were also significant distinction among groups treated with different time period. The H2O2, MDA content and active of anti-oxidant enzymes of Catharanthus roseus were both raised remarkably when the supplementary UV-B radiation or nitrogen nutrition existed separately or together during the initial period of treatment. The UV-B absorbing compounds were enhanced remarkably under the condition of supplementary UV-B radiation, and it was further increased when the nitrogen nutrition were added.3,There were noticeable effects of supplementary UV-B radiation and nitrogen nutrition on the photosynthesis characteristics of Catharanthus roseus, and there were also significant distinction among groups treated with different time period. The net photosynthetic rate, stomatal conductance and transpiration rate were all markedly reduced when the supplementary UV-B radiation or nitrogen nutrition existed separately during the initial period of treatment, however, comparing with single treatment, the net photosynthetic rate and stomatal conductance were raised remarkably when both conditions existed. The water use efficiency was enhanced remarkably by the supplementary of nitrogen nutrition, but it was reduced in the supplementary of UV-B radiation. The total chlorophyll and carotenoids content were all increased notably under the condition of long term treatment of supplementary nitrogen nutrition, but the total chlorophyll and carotenoids content were all decreased notably when supplementary UV-B radiation was added, or when it existed together with supplementary nitrogen nutrition in the long term treatment.4,There were noticeable effects of supplementary UV-B radiation and nitrogen nutrition on the vindoline, catharanthine and vinblastine content in Catharanthus roseus. The three alkaloids contents were all increased markedly under the condition of supplementary UV-B radiation and nitrogen nutrition, comparing with treated separately with either of them. Based on the apparent positive correlative existed among the three alkaloids contents, we supposed that the increase of the three alkaloids content may be mainly resulted from the biosynthesis of the precursor utilizing the supplementary nitrogen source.