After Erythronium japonicum seed recovery storing, has does not geminate, it is a question of the seed multiplication technology. This article picks the Erythronium japonicum seed biology characteristic and stores in the process the nutrients content, the enzyme activity and the conductivity change, the research seed biology characteristic as well as the reason after picking the seed appear difficultly to sprout, then the understanding seed vital activity rule and the germination mechanism, explore the promotion seed geminate method by use the utilization different temperature and medicament processing, this research recovers the seed from 2007 to 2009 to divide into the three kinds:1. Erythronium japonicum seed's biology characteristic:the seed is the small-sized seeds; After picking, under the different age and the different storing condition, the 1000-seed weight, the relative water content, the viability have differently. After picking, natural dry up 30d by airing, the 1000-seed weight of A group seeds was 7.14g, the relative water content was 15.42%, the water content is high, the viability reaches 83.7%. After picking, the humidity environment short time preservation's B group of seed part has the germination, the seed internal nutrients and the moisture content consumption, the 1000-seed weight and viability, the water content as well as the nutrients content is lower than A. The 1000-seed weight of B group seeds was 6.42g, relative water content was 18.07%, the viability was 78.2%.2. A, B group of seed nutrients content difference is not big, the soluble sugar content respectively is:8.15%,8.25%:The starch content distinguishes 56.70%,58.69%; The soluble protein content is 6.09mg/g,6.23mg/g; And the seed water permeability is good, the vigor difference is not big. Therefore seed does not sprout and the water permeability, the nutrients content as well as the vigor factor relevance is not big. seed storage process, nutrients content, enzyme activity and cell membrane penetrable dynamic change:The soluble sugar and the starch content have the declining trend; The soluble protein content is stable, has the slow rise slightly, and the change is not remarkable; The peroxide enzyme activity assumes the drop slow tendency; the catalase activeness slowly rises; a-amylase activity changes not obviously;β-amylase activity assumes the slow rise the tendency, changes not obviously; The seed conductivity presents the rise the tendency, all targets have certain change, but changes is not remarkable, in the seed storage process, the starch transforms the soluble sugar, the sugar decomposes altogether vital activities and so on seed breath, seed internal four kind of enzyme activeness and the conductivity is low, and the change is not remarkable showed that in the seed the physiological biochemistry change degree is small, therefore after seed storage, the nutrients content and the enzyme activity cause the reason which the pig tartar seed difficult to sprout.3. After seed in June mature recovery, the part has the germination characteristic which under the moisture content sufficient condition the obvious radicle exposes one's valuables, exposes one's valuables it with has not exposed one's valuables the seed open ground direct seeding, next year in mid-May the normal germination, the germination percentage will be 7.3%, pig tartar available seed multiplication.4.The seed various part of water immersion fluid exists suppresses the cabbage seed germination and the growth material, whether these materials do affect pig tartar seed own germination to wait for further studying.5. With different density's GA3 and 6-BA and so on biological activity materials as well as the medicament processing seed does not have the germination phenomenon, explained that the broken dormancy function does not have the influence to the seed germination. seed dissection indicated that has the complete embryo's shape structure, B group has the germination phenomenon, explained after pig tartar seed storage, does not sprout and the physiological additional ripening and the dormancy does not have the relevance.6. The seed storage experiment gets moldy and spoil suppresses is most effective by 0.5% KMnO4 solution immersion disinfection effect; seed tends to mould in 25℃constant storing; 4℃under the condition, different density's GA3 and 6-BA processes seed getting moldy and spoil rate is low, and GA3 processes the seed quality is good, getting moldy and spoil rate are extremely few. |