| Plant is often exposed to biotic and abiotic stresses of varying degrees in growth process which affected plant growth and development badly and cause huge negative effect to agriculture. Peanut is an important oil and cash crop. China is one of the most vital peanut-cultivated countries in the world. Peanut distributes mainly in arid and semi-arid regions. Drought significantly affects the yield and quality of peanut. It is reported that 70% of peanut area in China are in various degree of drought and the average decrease in yield was more than 20%. Therefore, it is essential to resolve the negative effect of drought stress to peanut production.The proteomics can be implied in many fields such as the protein expression level,the sequence of amino acids, the post-translational modification, the protein location and the protein-protein interaction, which cannot be solved by genomics. The proteome of many plants, such as Arabidopsis, rice and corn, has been studied. The physiological changes under various stresses, such as drought, high salt content, hypoxia, ABA treatment, and mechanical damage, have been investigated. Influence on plant growing by biologic factors such as mycorrhizal fungi and plant accompanied rhizobium, changes of protein in the growing process of plant's tissue such as root, stem, leafe, fruitage, and seed. Proteomics has also extended its studying field to sub-cell level, meaning to study proteome inside organelles including chloroplast, mitochondria, Internal membrane system etc. Some other fields are also studied in Proteomics, such as the relationship between genotypic diversity and phenotypic diversity, protein diversification caused by gene mutation, mechanism of phytohormone and so on.The results show that both quantitative and qualitative changes of many proteins are induced under these stresses. Moreover, the study of plant proteomics can be conducted at the subcellular level.Peanut is an important oil crop and possess high nutritional value, it's a kind of allotetraploid plant. The study of peanut at the genomic level, however, is limited due to its huge genome. The peanut proteomics is also at an initial stage.In the present study, leaf soluble proteins were extracted from a kind of peanut (Luhua 14) and were analyzed by dielectrophoresis. Well reproduced dielectrophoresis spectra with a large number of protein spots were obtained on the base of precipitation method using trichloroacetic acid and acetone as solvents. Sample extraction, protein dissolution, range of pH, protein amount, SDS-polyacrylamide gel electrophoresis and other key steps were improved. Better dielectrophoresis spectra were obtained when 280μg of protein was used and the pH of IPG adhesive tape was 4-7. About 480 protein spots were observed clearly from the 2-DE spectrum while no obvious tailing phenomena was observed. 40 protein diversity were found according to analysis and comparation, 17 of them are up-regulation of experssion and 23 of them are down-regulation of experssion.Five differential proteins corresponding to drought stress were confirmed through MALTI-TOF MS measurements and data retrieval. Although the results caused by in-gel digestion are unsatisfactory, the presented in this study contributes to the peanut leaf proteomics.
|
PDF Full Text Request