The RNA Interference Vector Construction Of Gene With MATH Domain In Seed Development Of Brassica Napus And Transgenetic Study

Molecular regulation mechanism to synthesis of fatty acid was researched less in Brassica napus. A gene including MATH domain screened from EST of SSH Library about seed development in Brassica napus. Alignment showed that it had a high degree of similarity with Arabidopsis thaliana At3g20370, named BnaMT-1.Semiquantitative RT-PCR showed BnaMT-1 gene had different expression in Brassica oleracea, Brassica pekinensis and Brassica napus.The BnaMT-1 gene fragment with 327bp cloned was inserted to plasmid, with a seed specific expression NapinA promoter, to construct a BnaMT-1 gene RNA interference vector which was used to interfere the gene expression. The RNA interference vector was transferred into Brassica napus XY15 by Agrobacterium-mediated transfrormation.Transformants were screened by PPT resistance and PCR amplification.One positive transformation of seedlings were detected,which paved the way for further study on the function of BnaMT-1 gene in Brassica napus.