Wry Head Disease Of Bullfrog Pathogen,Pathology And Sensitive Drug Research

In recent years, it can be diagnosed as Bullfrog Wry Head Disease. Taking the symptoms into consideration that intumescent abdomen, darkening body, wrying head to one side, swim in rotating way, large amount of bloody pus dissection in its abdomen, tumid and dark purple liver, hyperaemia of kidney with fresh red color etc, appeard in Bullforg farms in Yunxi District of Yueyang City Hunan Province.Respectively abdomen, liver, intestine, kidney, stomach and other organizations of Bullforg extract the bacteria, separation, purification, identification, were carried out, and the result confirmed that Micrococcus luteus and Pseudomonas mendocina, this is the bullfrog Two New pathogen. The result of the best concentration screening test showed that:When the two kinds of pathogens according to 1:1, concentration of 1.1×109, its most pathogenic; The result of the best infection screening test showed that:form the eye injection so that incidence fast, and the same symptoms of incidence and nature. Therefore considered that Micrococcus luteus and Pseudomonas mendocina though the eye infection is obviously, and is pathogens of Bullfrog Wry Head Disease.The result of virulent factors constitute tests showed that:Micrococcus luteus and Pseudomonas mendocina in the Blood agar(37℃) cultured 24h, were no hemolysis ring, were negative; They and LAL reaction to form a solid gel, hold down fixed gel showed that endotoxin-positive; Injected at certain dose of healthy frog intestine, breeding 48h, observations show that exotoxin positive.Histopathological examinations of naturally occurring and experimentally infected cases were carried out. Important finds were inflammation, hyperemia, necrosis, and gangrene of local skin, as well as acute suppurative myositis, pneumonia, mild catarrhal gastroenteritis, hepatic necrosis, suppurative nephritis, acute aplenitis.Antibiotic sensitivity experiments showed that:The bacteria is highly sensitive to the 5 antibiotics, including a Cefoperazone, Levofloxacin and Fosfomycin, but not to the 13 antibiotics such as Chloramphenicol, Ciprofloxacin and Gentamicin in the 20 antibiotics. Therefore, there is no obvious affection a variety of antimicrobial drugs in the prevention and treatment. In addition, It is general difficult depth in, because the pathogen in the key parts of the brain and the eye and the brain barrier function. When the disease occurs, mortality rate will reach 95%, even up to 100%.The results mentioned above shown that the Wry Head Disease of Bullfrog was specific bacterial eye infections caused by infectious diseases, Related performance of the brain of Dead frog's disease to its exotoxin produced and the site of infection.