Molecular Evaluation Of Genetic Diversity And Cloning Of Chalcone Synthase Gene In Tartary Buckwheat

We studied the genetic diversity of 50 elite tartary buckwheat (Fagopyrum tataricum) germplasm by Amplified Fragments Length Polymorphism marker (AFLP) and Simple Sequence Repeat marker (SSR). Meanwhile, we had a comparison on effectiveness of two molecular markers systems. The results showed that 211 special AFLP markers were detected by 19 AFLP primer pairs and 160 special SSR markers by 19 SSR primer pairs. The Polymorphism Information Content (PIC) value was from 0.0241 to 0.1918 for AFLP and from 0.5908 to 0.9992 for SSR, respectively. The clustering analysis by Unweighted Pair Group Method with Arithmetic Mean (UPGMA) indicated that these germplasm resources could be divided into five clusters when the similarity coefficient was 0.790, and could be divided into four clusters when the similarity coefficient was 0.578.Although the results were not so consistent by the two kinds of markers, both of them were effective for studying tartary buckwheat genetic diversity. Moreover, we identified some good molecular markers primer pairs for F. tataricum, which will be very useful for assessing genetic diversity and identifying more appropriated varieties for breeding. Fianally, our work will also have a important reference on constructing more complete and detailed genetic linkage map, locating some important agronomic trait genes and determining the origion of F. tataricum.We cloned the key enzyme, chalcone synthase (CHS) gene responsible for synthesizing flavonoid in tartary buckwheat by Rapid Amplification of cDNA Ends (RACE), and got its Open Reading Frame (ORF).We analysed basic physicochemical properties and homology of the enzyme gene, and constructed the phylogenetic tree of gene family by neighbor-joining, which confirmed its evolution status and origin in F. tataricum.Our research provided a base on further study on its transform and functional testing. Moreover, it will paly an important role o molecular assistant breeding for the excellent F. tataricum varieties with high flavonoid content.