The Preliminary Study Of Biological Control In Edible Fungus Pathogenic Trichoderma Spp.

In this paper, mushroom cultivation in Fujian province to the 4 main types of isolated Trichoderma spp. species and 14 different edible fungi as experimental materials, selected on the antagonism of Trichoderma spp. antagonistic strong and weak even on non-edible fungi antagonistic bacteria 75 strains, which from the Bauhinia, aloe vera and banana peel were screened in bacteria 45, from the soil bacteria were screened 30 strains of Trichoderma spp. and effective screening of antagonistic 5 strains of bacteria in the number for the 5,31,126,179 and 258, Bacillus subtilis Bs-2 and Escherichia coli as CKs. Doing the physiological and biochemical tests for bacteria, bacterial identification manuals and references the BERGEY'S MANUAL OF SYSTEMATIC BACTERIOLOGY and Dong Xiu Zhu, Cai Miaoying, eds identification of common bacteria in the system manual to identify the results were Bacillus subtilis. The 5 strains. 16S rDNA molecular identification, the results of their sequencing on the NCBI GeneBank + EMBL + DDBJ + PDB gene library for BLAST homology analysis, identification results were Bacillus subtilis, and bacterial physiology biochemical tests were consistent with. Screening of bacteria with the same 4 species of Trichoderma spp. and 14 species of edible fungi as experimental materials, from fresh white garlic, fresh onions, licorice root, forsythia, honeysuckle, pomegranate rind, skullcap, Qin skin, berberine, rhubarb, summer subtilis, Elsholtzia, Money Grass, Banlangen, Schisandra, Andrographis paniculata, Polygalaceae, mint, Guanzhong, Agastache rugosa, clove, anise, cinnamon, tangerine peel, cuspidatum, leaves a total of 26 species of herbs were screened out of garlic, Agastache rugosa , licorice and cloves that four kinds of herbs, their relatively strong inhibitory effect of Trichoderma spp. different herbal medicine extracts on inhibition of Trichoderma spp. different, with a kind of herbal extracts, because of different extraction methods on wood There are also differences in mold inhibition. Mushroom in the herbal extract on the impact of the experiment, licorice root, Agastache rugosa, garlic different edible fungi, inhibit or promote different role, but also because of the different extraction methods, the same kinds of herbal extract on the same kinds of edible fungi have different functions.