Effects Of Modified Yupingfeng Pulvis On Nonspecific Immunity In Immunosuppressed Monopterus Albus

【Objective】The effect of the Chinese herb compound Modified Yupingfeng pulvis (MYP), which is composed by astragalus, atractylodes, divaricate saposhnikovia root, lanceolata and glycyrrhiza, on the change of nonspecific immunity in Monopterus albus was evaluated in this study.【Methods】All 198 Monopterus albus were divided into six groups,and solution was injected into abdominal cavity of each eel at the 3th day and the 9th day.The first group, acting as negative-blank control group,was just injected intraperitoneally with normal saline.The second group was injected with cyclophosphamide.One medication group was injected with cyclophosphamide and astragalus injection at the same time as drug-treated control fish.The other three groups were injected with cyclophosphamide and MYP simultaneously.Thirty-three eels were treated with 0.8μl/g·BW MYP,which was made up of Huangqi,Baizhu,Fangfeng,Dangshen and Gancao,as low-dosage-drug-treated fish, middle-dosage-drug-treated fish were injected with 1.6μl/g·BW MYP and the high-dosage-drug-treated fish was injected with 3.2μl/g·BW MYP.Counting of mortality rate and spleen index,and detecting the change of enzyme activities at the 15th day of experiment.Observating the organization form changes in the spleen and intestine.【Results】The results indicated that,compared with the masculine-blank control group, the mortality rates of each treated group were decreased,the level of spleen index was elevated,the activities of SOD,CAT and GSH-Px increased obviously,and the low-dosage-drug-treated group showed the best effect.Overall,these results as mentioned above demonstrated that MYP can enhance the level of spleen index,and also bring the activities of SOD,CAT and GSH-Px going up significantly.Compared with the positive-blank control group, the activities of LSZ, AKP and ACP increased obviously, and the low-dosage-drug-treated group showed the best effect. Overall, the results demonstrated that MYP can enhance the activities of LSZ, AKP and ACP significantly. The positive-blank control group appeared in corresponding changes in the organization form or even damaged. The splenic capsule becamed atrophic or even exfoliated.Splenic sinus and splenic cord was not clear, and the boundaries were obviously fuzzy.The number of lymphocytes reduced significantly and the cell nucleus were not clear.Observed that the nuclear membrane was cracked and part of the nuclear membrane was dissolved, the organelles such as endoplasmic reticulum and mitochondria were decreased and even not be observed.Shorter length of intestinal villi,larger Width of the between of the wide recess,at the same time there is a significant reduction in goblet cells.It suggested that the Chinese medicine preparation could improve the clinical performances and the anatomia pathological symptoms obviously in the Monopterus albus damaged liver.【Conclusion】The preparation has a effective immunopotentiator against the experimental immunosuppression of Monopterus albus.It can increased the activities of SOD,CAT,GSH-Px,LSZ,AKP and ACP obviously. MYP was proved to be a effective organs-protective medicine against the organs injury induced by cyclophosphamide.