Studies On The Effects Of Arbuscular Mycorrhiza Fungi On Growth,yield And Quality Of Sweet Pepper In Greenhouse

Arbuscular Mycorrhizal is a symbiosis by Arbuscular Mycorrhiza Fungi(AMF)and root of plants, which also is a kind of widespread natural phenomenon. AMF play an important role in improving absorption of nutrients of crops, expanding the scope of roots, promoting growth of plant, increasing the yield and improving quality of production after infected the plants. In this study, using a kind of sweet pepper named zhongjiao105 as experimental material, the AMF strains and substrates were screened which are suitable for cultivating sweet pepper Mycorrhizal seedlings. Then the effect of AMF on growth ,yield and quality of sweet pepper was studied in greenhouse, and also studied the structure and diversity of bacteria species in sweet pepper rhizosphere. This study aimed to provide a theoretical basis for production of high quality sweet pepper and exploiting the application value of AMF. The main results as follows:1. The peat vermiculite with low nutrition content is more suitable for cultivating Mycorrhizal seedlings. The colonization percentage of AMF to sweet pepper roots is high, GM is 66.5% and GV is 54.5%.There are obvious effects on sweet pepper seedling with AMF, such as the increasing in plant high and stem diameter; the root activity of sweet pepper with GM and GV are improved by 36.9% and 15.8%,the photosynthesis improved by 61.42% and 22.46%,the dry weight improved by 29.9% and 23.4%; The impact of GM is better than GV;The effect of AMF on sweet pepper seedling in organic and general soil are relatively less.2. AM fungi significantly improved the root activity of sweet pepper and promoted the germination of fibrous root; The chlorophyll content and photosynthesis of sweet pepper leaves markedly improved by inoculating AMF treatment, which are better in organic soil; Compared with control, the inoculating AMF treatment significantly increased the yield of sweet pepper by 13.6% in organic soil and 12.6% in general soil; The quality of sweet pepper was also improved by inoculating AMF treatment, which shown as the decrease of nitrate content and increase of total sugar, Vc and crude protein.3. There are some effects on the structure and diversity of bacteria species in sweet pepper rhizosphere with different treatment; There are some common bacteria species between different treatment, but there are some change in the number of these bacteria species; Besides, there are also some special bacteria species; These shown that the structure of bacteria species in both soil have same changes with the inoculation of AMF, which including the increase and decrease of some kinds of bacteria.4. The number of rhizosphere bacteria species in organic soil first increased and then decreased from planting prophase to full fruit period, but there is no change in organic soil; The number of bacteria species reduced with the inoculation of AMF in both soil and any period; The decrease of microbial kinds is only in the number through compare the DGGE profile.5. Compare with general soil, the similarity coefficient is bigger between each period in organic soil, which showed that the bacterial flora of organic soil are more stability than general soil without other interference; The similarity coefficient between inoculating AMF treatment and CK of general soil is bigger than that of organic soil in both periods, which showed that the effect of AMF on diversity and structure of soil microbial is stronger in organic soil than that of general soil.