The Synthesis Of Tetracyclines Molecularly Imprint Polymers And The Evaluation Of Their Recognition Characters

Tetracyclines are mainly used as drug additives in animal production. They could effectively prevent intestinal infection and promote aniaml growth. With a stable core chemical structure, the animal use tetracyclines and their metabolites release to the envrionmental system and can not be decomposed in a short period of time. The residue may induce the tolerance of bacteria and impact the whole environmental system. The concentration of tetracyclines residue in environment are extremely low and the environment sample preparation is a tedious and complicated process. In hence, how to establish a tetracyclines analysis method with ideal specialization and high sensitivity is critical to the evaluation of the environmental impact of these antibiotics residue.Currently most countries control the tetracyclines residues in animal products and several tetracyclines analysis methods are applied in real sample tests. Among them, LC-MS analysis is one of the most widely used method especially during the complicated environmental samples analysis. A proper prepared sample process determines a successful LC-MS analysis. However, unfortunately most sorbents in solid phase extraction are non-specific sorbents (eg. C18).Molecularly imprint is a newly developed technology in absorption science. Compared with other sorbents, molecularly imprinted polymer is tailored-make material with high specific absorption. And several MIP based molecularly imprint polymer solid phase extraction (MISPE) were developed to cope with different real samples.In this research, we used doxycycline as the template and polymerized the tetracyclines(tetracycline, oxytetracycline and chlortetracycline) absorption MIP by precipitation method. Then used the MIP as the SPE sorbent. We tested the maximum loading concentration and also discussed the loading condition and procedure of this tetracyclines MISPE. The main points of this thesis are as follows:1. We compared the methods in tetracyclines analysis. Then introduced the principle of molecularly imprint technology, the polymer preparation methods and the characterization of MIPs. In the end, the applications of MISPE were briefly reviewed.2. Polymerized the MIP by precipitation method. And the final reaction condition is 1mmol(0.4809g)deoxycycline,60ml acetonitrile,8mmol(0.68ml)MAA,16mmol(5.1ml)TRIM and AINB 45mg were mixed and reacted under 60℃for 24 hours with stiring. The absorption characterization and physical characteriztion of this MIP were conducted. And according to the IR validation the tetracyelines absoptive MIP were successfully synthesized and it shows good thermostability.3. Finally, we tested the maximum loading concentration and discussed the loading condition and procedure of this tetracyclines MISPE. A 0.4 mg maximum loading capacity was confirmed. And the final loading procesure is 5 ml methonal for activation, 10 ml sample in water for loading, 3 ml 5% formic acid in water for cleaning and 6 ml 1% formic acid in methanol for washing.