Proteomics On Eggs At Later Stages And The Newly-hatched Larvae Of Egg Color-limited Bombyx Mori Variety, W-1

Silkworm is a complete metamorphosis insect, In their lifetime they go through four different developmental stages:egg, larvae, pupa and adult .Protein of eggs in different periods have been studied. But protein of gender differences of eggs and newly-hatched larvae in Bombyx mori have little report .In this study, we identify different protein on eggs at later stages and the newly-hatched larvae combining two-dimensional electrophoresis and mass spectrometry using egg color-limited Bombyx mori variety (W-1),Furtherly we find that gender of the Bombyx mori have an influence on organs and tissues during embryonic development. Main results obtained are as follows:1 male and female embryonic morphology of egg color-limited Bombyx mori variety,W-1,in different periodsmale and female embryos of egg color-limited Bombyx mori variety,W-1 in different periods were successfully dissected with potassium hydroxide.Then prepare for the follow-up experiments.And for the first time male and female embryos are presented separately.2 The study of expression profiling of dissolvable protein of male and female eggs of the period of ji3 in Bombyx mori variety,W-12.1 The establishment of patterns of dissolvable protein of male and female eggs of the period of ji3 in Bombyx mori variety,W-1 by two dimensional polyacrylamide gel electrophoresiswe compared the dissolvable protein of male and female eggs of the period of ji3 by using two-dimensional electrophoresis technology. The results showed that 317 and 335 protein spots were detected in dissolvable proteins of male and female egg respectively. Comparison analysis showed that 273 of the total protein spots were matched between male and female egg, and 44 and 62 specific protein spots were found in male and female egg respectively. The results indicated that dissolvable protein of male and female eggs of the period of ji3 in silkworm are different between male and female.2.2 the identification of different proteins from patterns of dissolvable protein of male and female eggs of the period of ji3 in Bombyx mori variety,w-1 by mass spectrometryThe characteristic protein spots were excised to identify by MALDI-TOF MS.The result show that proteins identified were putative cuticlar protein, tropomyosin isoform 5, Bmsqd-1, transgelin , heat shock protein 20.4 ,tropomyosin 1 and Tm1 protein similar to Drosophila.It furtherly show that gender has an important influence on the formation of embryonic organs and tissues.3 Anaylsis of patterns of protein of male and female eggs of the period of ji4 in Bombyx mori variety,W-1 by two dimensional polyacrylamide gel electrophoresiswe compared the protein components of male and female eggs of the period of ji4 by using two-dimensional electrophoresis technology. The results showed that 486 and 427 protein spots were detected in proteins of male and female egg respectively. Comparison analysis showed that 380 of the total protein spots were matched between male and female egg, the matching rate is 81.29% and 106 and 47 specific protein spots were found in male and female egg respectively.4 Anaylsis of patterns of protein of male and female eggs of the period of ji5 in Bombyx mori variety,W-1 by two dimensional polyacrylamide gel electrophoresiswe compared the protein components of male and female eggs of the period of ji5 by using two-dimensional electrophoresis technology. The results showed that 471and 432 protein spots were detected in proteins of male and female egg respectively. Comparison analysis showed that 395 of the total protein spots were matched between male and female egg, the matching rate is 84.27% and 76 and 37 specific protein spots were found in male and female egg respectively. 5 The study of protein expression profiling of the male and female newly-hatched larvae in Bombyx mori variety,W-15.1 The establishment of protein patterns of male and female newly-hatched larvae in Bombyx mori variety , W-1 by two dimensional polyacrylamide gel electrophoresiswe compared the protein components of male and female newly-hatched larvae by using two-dimensional electrophoresis technology. The results showed that 478 and 444 protein spots were detected in dissolvable proteins of male and female egg respectively. Comparison analysis showed that 410 of the total protein spots were matched between male and female egg, the matching rate is 88.57% and 68 and 34 specific protein spots were found in male and female egg respectively.5.2 the identification of different proteins from patterns of male and female newly-hatched larvae in Bombyx mori variety,w-1 by mass spectrometryThe characteristic protein spots were excised to identify by MALDI-TOF MS.The result show that proteins identified were major plasma protein 30K, heat shock protein 21.4, low molecular 30kD lipoprotein PBMHPC-21, myosin light chain 2,putative protein CLK_A0112and GM17246 protein similar to Drosophila.It furtherly show that gender has an important influence on the formation of embryonic organs and tissues.