Role Of Microtubule Depolymerization Drug In The Resistance Of Wheat Against Wheat Stripe Rust And Cucumber Powder Mildew

Cytoskeleton is a new cellular structure which was found in the mid 1960s. Because it has many important physiological functions, cytoskeleton has been research frontiers since it was discovered 30 years ago, and the application of cytoskeletal depolymerization drug drives it to make a considerable progress. Pre-injecting microtubule depolymerization drugs oryzalin before inoculation, the effect of oryzalin on the hypersensitive cell death, H2O2 accumulation and papilla formation rate in wheat cells in the incompatible interaction of wheat (Triticum aestivum L.) cv.'Suwon 11'and Puccinia striiformis Westend f. sp. tritici Erikss., CY23, as well as non-host combination of non-host pathogen Sphaerotheca fuliginea, was studied by histopathological detection of HR, DAB staining and Coomassie brilliant blue staining. The results showed as follows:1. By HR histopathological detection to investigate the influence of microtubule depolymerization drugs oryzalin on hypersensitive cell death in host and non-host interaction systems. In incompatible interaction of wheat (Triticum aestivum L.) cv.'Suwon 11'and P. striiformis Westend f. sp. tritici Erikss., CY23, the infection site proportion of the hypersensitive cell death and extent in the oryzalin treatment was significantly lower than that of the water control. In the non-host combination of wheat and S. fuliginea , the infection site proportion of the hypersensitive cell death and extent in wheat epidermal cells in the oryzalin treatment decreased apparently compared with that of water control. The results showed that after injecting the microtubule depolymerization drug to the wheat leaves, the hypersensitive response in both incompatible interaction and non-host combinations was inhibited, indicating microtubule plays an important role in the resistance of host and non-host expression.2.Using Diaminobenzidine (DAB) histological chemical staining to investigate the effect of microtubule depolymerization drugs oryzalin on H2O2 accumulation in host and non-host cells . In the incompatible interaction between wheat and P. striiformis Westend f. sp. tritici Erikss., CY23, the DAB staining was detected in both the oryzalin treatment and water control, indicating that the accumulation of H2O2 occurred in both treatment and control. But the infection site proportion of the accumulation of H2O2 in the oryzalin treatment was significantly lower than that in the control. So were the change during the interaction between wheat and nonpathogen S. fuliginea . In this two interaction systems, the area of DAB staining is smaller and lighter in the treatment than in the control, which indicated the less H2O2 accumulation induced by the pathogens. The results showed microtubule may be related to the ROS burst induced by the pathogens in host and non host cells.3.With Coomassie brilliant blue staining, the impact of microtubule depolymerization drugs oryzalin on papilla formation in non-host interaction system was studied. In the interaction of wheat - S. fuliginea , we observed the papilla formation in treatment and control,and found that it had no significant effect in injecting microtubule depolymerization drugs.