Effects Of Dietary Addition Of ω-3 PUFA On Laying Performance, Lipid Metabolism And Immune Function For Shao Ducks

The exploration of functional animal food is a key content in the field of animal nutrition. Being rich in co-3 polyunsaturated fatty acid (co-3 PUFA), fish oil is a good source of unsaturated fatty acid. In the present study, seven hundred and twenty 300-day-old Shaoxing ducks were randomly allocated to 6 blocks of 120 ducks (4 replicate), with 40 ducks of each replicate, based on a similar body weight (1560g) and were fed one of 6 diets:basal diet (control,145A), basal diet and 1%fish oil (treatment 1),2%fish oil (treatment 2),3%fish oil (treatment 3),2%fish oil and VE (50PPM, treatment 4),2%fish oil and dihydropyridine (200ppm, treatment 5). Laying performance, immune response, and lipid metabolism were measured for the appropriateω-3 PUFA level in the diet. The trial lasted for 4 weeks.Compared with control, ducks fed dietary treatment 1, treatment 2, and treatment 3 increased laying rate by 4.00 (P>0.05),7.04 (P<0.05),and 4.71%(P>0.05), respectively; decreased egg-feed ratio by 1.72 (P>0.05),8.28 (P<0.05), and 8.97% (P<0.05), respectively; slightly improved egg weight (P>0.05). Laying rate and egg-feed ratio were unaffected between treatment 2 and treatment 3 (P>0.05). There were no effects of daily feed intake, broken egg rate, and elimination rate among 5 treatments (P>0.05).Relative to control, treatment 2 and treatment 3 increased DHA by 9.70 (P<0.05), 9.33 times (P<0.05); increased EPA by 3.57 (P<0.05),2.66 times (P<0.05). Eggs for treatment 2 and treatment 3 had higher yolk ALA level relative to control (P<0.05).Treatment 2 reduced egg cholesterol by 28.25%(P<0.05)and MDA by 22.68% (P<0.05) compared with control.Serum IgG, IgA, and IgM for treatment 2 was 9.76 (P>0.05),29.66 (P>0.05), and 37.12%(P>0.05) higher than control.Treatment 2 and treatment 4 increased yolk DHA, EPA, and ALA by 62.28 (P<0.05),30.85 (P<0.05), and 42.35%(P<0.05), respectively, relative to control. Meanwhile, the yolk cholesterol and lipid peroxide were 39.41 (P<0.05) and 17.15% (P<0.05) lower for treatment 2 and treatment 4 compared with control.Treatment 2 and treatment 5 increased yolk DHA, EPA, and ALA by 52.94 (P<0.05),22.89 (P<0.05), and 37.47%(P<0.05), respectively, relative to control. Meanwhile, the yolk cholesterol and lipid peroxide were 49.76 (P<0.05) and 20.14% (P<0.05) lower for treatment 2 and treatment 5 compared with control.Treatment 1,2, and 3 resulted in 11.74,53.48, and 21.30%higher economical profit than control.It is suggested from the above-mentioned results that dietary inclusion ofω-3 PUFA may increase laying performance and improve lipid metabolism and immune function. In summary,2%fish oil supplementary is appropriate.