The Oviposition Deterrent Effect Of Secondary Materials From Xanthium Sibiricum (Compositae) On Plutella Xylostella And Their Chemical Compositions

Biological activities of secondary substance from Xanthium sibiricum on Plutella xylostella were studied. The different components were isolated from X sibiricum by using active tracking methods,and highly active compounds were further separation, purification and biological testing. Antifeedant, oviposition deterrent activity of highly active ingredients were studied on lavae of P. xylostella, and choice of trending action of the P. xylostella adults were recorded. Finally, the chemical composition of highly active components were analyzed by GC /MS technology. The main findings are as follows:(1)The extraction,separation and activity tracking test of secondary substance from Xanthium sibiricumExtracted by Soxhlet extraction Xanthium,will be the methanol extract of Xanthium extraction with chloroform,on Xanthium extracts were separated by column chromatography to obtain crude extracts of five fractions,Antifeedant activity assay of crude extracts was found in the concentration of 0.1mg/ml four components, the activity against Plutella up 81.29 percent, far more than other components.Determined using crude extracts of the adult moth's oviposition activity measured four components of the crude Oviposition activity was 63.65%, other components are at 37%or less. Active tracking results show that Xanthium extracts, the highest activity of crude fractions 4(2) the active component of Xanthium further separation,purification and biological activity determinationComponent 4 will be conducted a number of column chromatography and recrystallization in 12 fractions obtained crystallization method using leaf butterfly activity test,results found: fraction 10 and fraction 11 antifeedant activity and oviposition activity was significantly higher than other fractions of Plutella activity with the increase of concentration,while the other fractions of antifeedant was no significant features of the test when the concentration of 0.1mg/ml antifeedant rate were 79.78%and 82.15%. In the diamondback moth oviposition activity of selective determination, fraction 11 was the best, 24h,48h,72h of oviposition rate was 87.03 percent,75.75 percent, 68.79 percent, the study found that oviposition activity effect gradually decreased with time. Fraction 11 and fraction 10, the biological activity lasted significantly longer than that of azadirachtin.(3) Xanthium active fractions affect the behavior of the diamondback mothY-olfactometer used to test active fractions of the diamondback moth adults Xanthium behavior choice of trend was found:fraction 10, fractions 11 and Azadirachtin on the unmated female and male moth has a significant negative trend role in the control group the difference was very significant, which did not sire selection coefficient of diamondback moth azadirachtin minimum, up to 19.72%, composition 11, reached 24.17%, not mating female diamondback moth The relative selectivity coefficient of 11 fractions of the highest, reaching 23.89%. Unmated male, female moths, mating female moths on the choice of 10 fractions of coefficients were 28.06%,25.56%,29.44%, and 11 are very similar fractions. Moth relative to other fractions selection coefficient is not obvious. Diamondback moth is also found that male and female, whether mating did not affect the active component of the negative trend Xanthium response.The emergence of the diamondback moth 1 day into the insect rearing cage to observe the spawning behavior of the diamondback moth was found:the control group after 12 hours there is a small amount of moth eggs in the leaf veins depression, three days after the moth a large number of products egg,7 days a yellow patches of egg masses gathered there, while the treatment group of 11 fractions, only 24 hours after the moth eggs, insect rearing cage and the walls of the top of the eggs appear to be more than the control group much concentration of Chinese cabbage leaves no yellow patches of egg masses, suggesting that the inhibition of composition of 11 eggs in Chinese cabbage moth activity.(4) Xanthium active component of the GC/MS analysisThe fractions 10,11 to GC/MS analysis,fractions 10 and 11 contain fractions with Xanthium sitosterol,stigmasterol,and a small amount of vegetable oil sterol,sitosterol which Xanthium,Xanthium richness Stigmasterol high,the fraction 10 with fractions 11 has similar biological activity,and antifeedant activity,oviposition activity and the activity of the negative trends to be slightly lower than the 11 fractions, may now Xanthium Xanthium sitosterol and stigmasterol content ratio, the number of relevant content.The paper on the active substance several times Xanthium isolated the active component and active component of the impact of insect behavior,look under the plant's chemical defenses and insect behavior co-evolution theory,developed in order to function as repellent Lord, antifeedant and insecticidal deputy's new "plant protection agent."...