Effects Of Lipid Nutrition On Growth And Reproductive In Male Redclaw Crayfish, Cherax Quadricarinatus

Effects of lipid nutrition on growth and reproductive in male redclaw crayfish (Cherax quadricarinatus) were studied by the examination of bio-chemistry, histological and enzymatic methods. First we from the perspective of dietary lipid sources on growth of male Cherax quadricarinatus and found the best fat source suitable for growth. In particular fat source of dietary, we add different cholesterol levels in feed, study its effect on growth and reproductive in gonad rapid development phase. According to fill the research blankness of shrimp nutrition and metabolism of cholesterol in the diet of optimal addition, in order to resolve the shrimp cultivation practice in breeding prawn training problem and provides theoretical support for the research of full price plus feed.1 Influence of different lipid sources on growth, fatty acid composition and digestive enzyme activity in male redclaw crayfish, Cherax quadricarinatus.The the fatty acid composition of hepatopancreas and muscle, and the activity of hepatopancreas digestive enzyme of Cherax quadricarinatus with the body length of 11±1.05 cm that fed with commercial feed and four kinds of diet with different lipid sources (fish oil, peanut oil, soybean oil, pork lard) respectively after 8 weeks breeding were analysed. The results indicated that the highest weight growth rate and feed rate were detected in the Cherax quadricarinatus fed the diet with soybean oil as the lipid sources, the lowest weight growth rate, feed rate and survival rate were detected in the Cherax quadricarinatus fed the diet with pork lard as the lipid sources. The main profiles of the fatty acid of Cherax quadricarinatus were 16:0,18:1 n-9 and 18:2 n-6, but the content of fatty acid were different. The highest composition of SFA was detected in the Cherax quadricarinatus fed the diet with pork lard as the lipid sources (38.10%) and the lowest was detected in the Cherax quadricarinatus fed the diet with soybean oil as the lipid sources(27.78%). The highest composition of MUFA was detected in the Cherax quadricarinatus fed the diet with peanut oil as the lipid sources (39.55%) and the lowest was detected in the Cherax quadricarinatus fed the diet with soybean oil as the lipid sources (29.78%). The highest composition of PUFA was detected in the Cherax quadricarinatus fed the diet with Soybean oil as the lipid sources (42.43%) and the lowest was detected in the Cherax quadricarinatus fed the diet with lard as the lipid sources (24.45%). The lipdase activity of Cherax quadricarinatus fed the diet with Soybean oil as the lipid sources was significant higher than that of peanut oil, fish oil and pork lard but significant lower than that of control grup. The Trypsin activity of Cherax quadricarinatus fed the diet with Soybean oil, peanut oil and fish oil has no significant different but significant higher than that of pork lard. The amylase and cellulase activity among the Cherax quadricarinatus fed the diet with different lipid sources has no significant different. In conclusion, the Cherax quadricarinatus fed the diet with peanut oil as the lipid sources has better growth rate and survival rate than other lipid sources, that may be caused by the special fatty acid rate just like EPA/ARA/DHA and the composition of HUFA has met the nutrition needs of gonad accelerated phase of Cherax quadricarinatus.2 Effects of different levels of cholesterol on growth, bio-chemistry composition and digestive enzyme in male redclaw crayfish, Cherax quadricarinatusThrough the initial body length 10.5cm in the male redclaw crayfish (Cherax quadricarinatus) fed cholesterol for 10 weeks with different artificial diets (the control and experimental group 1 to 4 groups of cholesterol were added,0,0.25%, 0.5% 0.75%,1.00%), shrimp in each group were measured in bio-chemistry composition and hepatopancreas digestive enzymes. The results showed that Group 2 shrimp had the best growth index, group 3 had the highest crude fat content, group 2 had the highest crude protein content. The hepatopancreas of linoleic acid and ARA (20:4 n-6) content with cholesterol addition rises; EPA (20:5 n-3) and DHA (22:6 n-3) content in the feed cholesterol level is 0.5% of the maximum; muscle fatty acid composition of various experimental group has no significant differences. Trypsin to experiment 2 group maximum, lipase activity there was no significant difference between group 2,3,4, but set higher experiment to group 1 and control. The results suggest that gonadal rapid developmental phase male Cherax quadricarinatus added to 0.50% of the cholesterol in feed can effectively promote the growth of the shrimp. 3 Effects of different levels of cholesterol on testis AKP, LDH and ATPase activity in male redclaw crayfish, Cherax quadricarinatus.Through the initial body length 10.5cm in the male redclaw crayfish(Cherax quadricarinatus) fed cholesterol for 10 weeks with different artificial diets (the control and experimental group 1 to 4 groups of cholesterol were added,0,0.25%, 0.5% 0.75%,1.00%), shrimp in each group were measured in testis AKP, LDH, ATPase activity. The results showed that testis AKP activity and LDH activity all add bloat rendering first by a downward trend, with the highest experiment 3 group, the experimental group AKP activity LDH activity are significantly higher than control group; testis ATPase activity there has no significant differences between group 2 and 3, but significantly higher than both experiment 1 to 4 group and control group. The results suggest that gonadal rapid developmental phase male Cherax quadricarinatus added to 0.50-0.75% of the cholesterol level in feed, the amount to be effective in promoting the development of shrimp gonad.