Genetic Transformation Of Maize With The Glyphasate-resistant Gene Mediated By Agrobacterimu Tumefaciens

Maize is an important food, feed, industrial raw materials for processing crops in the world. Weed damage is an important reason for yield reduction in maize. Glyphosate was served as the world's largest amount of a broad-spectrum herbicide and played a great role in protecting weed damage. Transferring glyphosate-resistant gene epsps into maize to cultivate glyphosate-resistant maize by genetic engineering method, it has a very important significance in weed control for corn production. In this study high efficiency binary expression vector was constructed based on pCAMBIA3301 vector, modifying by maize ubiquitin promoter, transit peptide of Arabidopsis thaliana chlorophyll (Ctp) gene, resistance to glyphosate Epsps gene components. The recombinant plasmid was transferred into Agrobacterium tumefaciens LBA4404 by freeze-thawing method, then transformed into maize immature embryos. The plants resistant to glyphosate in non-antibiotic marker gene were obtained. The preliminary identification in the field demonstrated that the transgenic maize plants were capable of resisting glyphosate's impacts to some extent.