Preliminary Study On FJ-08 Against Mycogone Perniciosa Of Agaricus Bisporus

Isolated a biocontrol bacteria FJ-08 against Mycogone perniciosa which be found in the culture process.The strain are antagonism to three groups of Mycogone perniciosa which are widely distributed in Fujian. The strian was identified by observation of the strain's morphology characteistics, physiological and biochemistry experiment, 16SrDNA sequence analysis and VITEK automatic identification of microbiology instrument.The identification results indicated the strain is Bacillus subtilis.The results of the culture condition indicated: The logarithmic growth phase was from 30h to 70h, 72h later entered its decline phaseThe optimal temperature for growth was 37℃and the optimal initial pH growth varies from 6.0 to 8.0 for strain of FJ-08 . FJ-08 can grow well when inoculation amount was 7%. The optimum volume of medium is 100ml/250mL. The result of four factors and three levels intersection experiment illustrated,the optimal culture condition of FJ-08 were temperature 37°C, pH 6.5, medium capacity 125mL/250mL, inoculation rate 9%. The major influencing factor is temperature and pH, and the temperature has greater influence.The study on nutritional condition indicated, FJ-08 could use organic nitrogen better, and the yeast extract were the best utilized nitrogen nutrition。D-galactose was the best utilized carbon source of FJ-08, but took the resource and performance price ratio into consideration, used sucrose as the carbon source was more suitable. The best concentration of yeast extract was 7.5%, and the best concentraion of sucrose was 6.5%.Result of LC-MS detection of crude extract of FJ-08 , speculte that the useful composition in extract may be surfaction and iturim.Effected mycelium growth on Mycogone perniciosa and Agarigus bisporus by different processing means showed, methanol had great influence on mycelium growth of As.0002+1. As the additive amount increased , the mycelium growth became shorter and shorter. The mycelium could hardly grow when it reach X4(added 2mL methanol in culture medium). In addition, the mycelium of As.0002+1 grew better in D2(added broth in culture medium) than in D1(added methanol in culture medium), it could further illustrated methanol affected badly on As.0002+1. Compared D2 and D3(added extract in culture medium), both of them caused significance influence on the mycelium growth of As.0002+1. However, D2 had stronger inhibition to the mycelium growth of M.p.001 than As.0002+1. Otherwise, D2 obviously inhibited the mycelium growth of Mycogone perniciosa, althrough the influence of mycelium growth of Agarigus bisporus reached significance level, the bactericide rate of Agarigus bisporus was far less than Mycogone perniciosa. Therefore, for prevention and cure of Mycogone perniciosa, selected D2 was the most suitable processing. Thus, When its concentration reached X1 (added 0.5mL broth in culture medium), the inhibition of it against Mycogone perniciosa attained obviously, meanwhile, it had low influence on Agarigus bisporus.In the cultivation of As.2796, the rate of three type of Mycogone perniciosa infected As.2796 were different for a same treatment. In all the treatments, the infection rate of three type of Mycogone perniciosa against As.2796 were also different. In Additon, three treatments caused three type of Mycogone perniciosa had much lower infection rate against As.2796 than control treatment. As.2796 had lower infection rate than others by using extact, and the highest was using by methanol. Meanwhile, after the three treatments, the rate of Mycogone perniciosaⅠ,Ⅱ,Ⅲinfected Agarigus bisporus existed significant difference. Agarigus bisporus cultivated by the treatments which mentioned above, whether had some bad problem to health. It needed to make further research, in order to make better use it in biocontrol of Agarigus bisporus.