Effects Of Fiber Enzymes On Nutritions Of Sugarcane Tip

The experiment taked the sugarcane tip,the important byproduct of sugar industry in Fujian Province,as the research object, had studied the cellulase, the xylanase and the pectinase to the sugarcane tip hydrolisis effect and the ensilage quality influence separately, based on this had discussed the formula of cellulase, xylanase and pectinase to the sugarcane tip hydrolisis effect and the ensilage quality, thus to provided the theory basis to enhance the use of sugarcane tip and the reasonable use of fiber enzymes.When pH value was 4.8, temperature was 50℃, sugarcane tip hydrolisised 8 hours separately by the cellulase, the xylanase and the pectinase. The result indicated that: 1. the cellulase, the xylanase and the pectinase hydrolysis sugarcane tip all can improve the feeding value of sugarcane tip by increasing the reducing sugar output and decreaseing the NDF and ADF content. The optimal usage of the cellulase, the xylanase and the pectinase respectively was 3.00 IU/g, 28.00 IU/g and 9.00 IU/g. When the addition of cellulase was 3.00 IU/g, the reducing sugar output was 8.44 mg/mL, sugarcane tip's NDF and ADF content were lower than that in the control group by 16.04%(P<0.01) and 9.69%(P<0.01). When the addition of xylanase was 28.00 IU/g the reducing sugar output was 7.98 mg/mL, the NDF and ADF content were lower than that in the control group by 9.67%(P<0.01) and 10.30%(P<0.01). When the addition of pectinase was 9.00 IU/g, the reducing sugar output was 4.54 mg/mL, the NDF and the ADF content were lower than that in the control group by 6.73%P<0.01)and 7.56%(P<0.01). 2. Compound cellulase hydrolysised sugarcane tip can improve the feeding value of sugarcane tip by increasing the reducing sugar output and reducing NDF and the ADF content, and shows better effect than that of the single enzyme on hydrolysis. When the cellulase, the xylanase and the pectolase recruitment were 4.00 IU/g, 35.00 IU/g and 9.00 IU/g, the reducing sugar was the highest-15.79 mg/mL; NDF and the ADF content were the lowest, lower than that in the control grou by 32.80%(P<0.01) and 28.95%(P<0.01).Ensilaged sugarcane tip 40 days by fiber enzymes. The result indicated that: 1. The addition of cellulase, xylanase and pectinase ensile the sugarcane tip separately, can improve the ensilage effect, the fermentation quality and the feed value. The optimal usage of the cellulase, the xylanase and the pectinase respectively was 3.00 IU/g, 21.00 IU/g and 9.00 IU/g. When the addition of cellulase was 3.00 IU/g , the pH value, the NH3-N content, the gas loss and the NDF and ADF content lower than that in the control group by 6.28%(P<0.01), 33.56%(P<0.01), 49.58%(P<0.01), 11.78% (P<0.01) and 7.48%(P<0.01) respectively. When the addition of xylanase was 21.00 IU/g the pH value, the NH3-N content, the gas loss and the NDF and ADF content lower than that in the control group by 4.59%(P<0.01),29.53%(P<0.01),43.21%(P<0.01),11.65%(P<0.01) and 7.43%(P<0.01) respectively. When the addition of pectinase was 9.00 IU/g the pH value, the NH3-N content, the gas loss and the NDF and ADF content lower than that in the control group by 2.44%(P<0.01),23.49%(P<0.01),44.88%(P<0.01),8.49%(P<0.01) and 3.47%(P<0.01) respectively. 2. Compound cellulase ensile the sugarcane tip separately, can improve the ensilage effect, the fermentation quality and the feed value. The formula of cellulase, xylanase and pectinase (4.00 IU/g, 21.00 IU/g and 12.00 IU/g) shows the lowest pH value and the NDF content, and lower than that in the control group by 8.94%(P<0.01) and 13.85%(P<0.01). When cellulase, xylanase and pectolase recruitment were 4.00 IU/g, 28.00 IU/g and 6.00 IU/g combination ensilage sugar cane tree top the ADF content is the lowest, reduced 8.98%(P<0.01) compared to the control group.