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Study On Biological Control Of Xyrosaria Lichneuta Meyrick Of Tripterygium Wilfordii Hook. F.

Posted on:2011-11-12Degree:MasterType:Thesis
Country:ChinaCandidate:J L ZhangFull Text:PDF
GTID:2143360305490975Subject:Forest Protection
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Xyrosaria lichneuta Meyrick belongs to the Lepidoptera,Yponomeutidae.It is one kind of new harmful insect about Tripterygium wilfordii Hook.F..The larvae eats the leaves of Tripterygium wilfordii Hook.F., already has had the significant impact on the growth and economic efficiency of Tripterygium wilfordii Hook.F..The study of the biological control on Xyrosaria lichneuta Meyrick isn't reported.In order to get the biological control program for Xyrosaria lichneuta Meyrick and provide scientific reference for Tripterygium wilfordii Hook.F. industrial,the biological test on Xyrosaria lichneuta Meyrick were carried out in Taining county,Fujian province between 2008 and 2009.1. Studies on Applying Beauveria bassiana and Metarhizium anisopliae to control Xyrosaria lichneuta Meyrick on Tripterygium wilfordii Hook.F.Control effects of Beauveria bassiana and Metarhizium anisopliae to the larvae of Xyrosaria lichneuta Meyrick were tested in the laboratory ,and all showed pathogenicity to Xyrosaria lichneuta Meyrick of Tripterygium wilfordii Hook.F. larvae.The data indicated that there were significantly difference at the toxicity to larve of Xyrosaria lichneuta Meyrick. The Bb27 caused the greatest mortality in 3 strains of Beauveria bassiana. The revised mortality rate of 1×107 spores·mL-1 among 12 days was 80.95%,and the LT50 was 5.10 days.The MaZPTP01 caused the greatest mortality in 3 strains of Metarhizium anisopliae. The revised mortality rate of 1×107 spores·mL-1 among 12 days was 78.57%, and the LT50 was 6.04 days.At room temperature,by spraying Xyrosaria lichneuta Meyrick with different concentrations, Beauveria bassiana Bb27 caused the greatest mortality in the 1×108 and 1×109 spores·mL-1,the larvae mortality was 100%,100%, LT50 was 3.34 days,3.05 days, LC50 was 6.7×104 spores·mL-1 among 15 days.Only Metarhizium anisopliae MaZPTR01 did not exceed 1×109 spores·mL-1,until the larve mortality was 100%, LT50 was 3.09 days, LC50 was 6.1×104 spores·mL-1 among 15 days. By using the Beauveria bassiana Bb27 and the Metarhizium anisopliae MaZPTR01 to control Xyrosaria lichneuta Meyrick larvae in the field.The results showed that the control efficacy reached 86.51%,83.98% by using suspension and power fireworks of the Bb27.The control efficacy reached 87.91,84.93% by using suspension and power fireworks of the MaZPTR01.2. Studies on Applying Entomopathogenic Nematodes to Control Xyrosaria lichneuta Meyrick on Tripterygium wilfordii Hook.F.The effects of four strains of Entomopathogenic nematodes on Xyrosaria lichneuta Meyrick of Tripterygium wilfordii were investigated in the indoor and outdoor and forest.The results showed that the infectivity of the nematodes was strongest at 24~30℃.The data indicated that the stain S. sp.CB2B displayed the strongest pathogenicity.The revised mortality rate of 100,250,500,1 000 and 2 000 nematodes·mL-1 among 6 days were 92.86%,100%,100%,100% and 100%.When the dosage reached 1 000 nematodes·mL-1,the insect larvae began to die on the first day and the indoor pest larva mortality rate among 5 days reached 100%. The Xyrosaria lichneuta Meyrick larvae and pupae were infected . The control effect was 88.15% in the forest experiment3. Studies on Applying Bacillus thruringlensis-Abametin and Bacillus thuringiensis to control Xyrosaria lichneuta Meyrick on Tripterygium wilfordii Hook.F.Control effects of Bacillus thuringiensis and Bacillus thruringlensis-Abametin to the larvae of Xyrosaria lichneuta Meyrick were tested in the laboratory and in the forest.The results showed that Bacillus thruringlensis-Abametin and light calcium carbonate mixed at the proportion of 1:10 and 1:15 could kill 96.00% and 92.00% of the larvae of Xyrosaria lichneuta Meyrick among 6 days in the laboratory. By using Bacillus thuringiensis to control the larvae,the revised mortality rate of 1:200 and 1:300 times all were 100% in the laboratory.The average decreased rates were 86.13% and 92.89% and the control effect reached 85.68 and 92.66% in the forest.The best choice is to use Bacillus thuringiensis 1:300 times and Bacillus thruringlensis-Abametin 1:10 in the forest, which can not only take a good control effect but also reduce the costs.4. Studies on Applying 1.2% nicotine and matrine EC to control Xyrosaria lichneuta Meyrick on Tripterygium wilfordii Hook.F.Control effects of 1.2% nicotine and matrine EC to the larvae of Xyrosaria lichneuta Meyrick were tested in the laboratory,the results showed that 1.2% nicotine and matrine EC had better control effect to the larvae of Xyrosaria lichneuta Meyrick. The larvae mortality was 100% in 24 hours at 1 000 times,the larve mortality mortality was 100% in 48 hours at 1 500 times,the larvae mortality was 1000 in 96 hours at 2 000 times.The toxicity of 1.2% nicotine and matrine EC could last 6~7 days.The rate of population reduction were 100%,100%,96.69% at 1 000,1 500,2 000 times .The control efficacy were 100%,100%,96.56% at 1000,1500,2000 times in the forest.The mortality peak was in the 2~3 days. The pesticide of 1.2% nicotine and matrine EC had specific efficacy to Xyrosaria lichneuta Meyrick,which were not harmful effects to the natural enemies and other insects.The best choice is to use 1 500 times to control in production practice,which can not only take a good control effect but also save the cost.
Keywords/Search Tags:Xyrosaria lichneuta Meyrick, Tripterygium wilfordii Hook.F., biological control, Entomopathogenic fungi, Entomopathogenic nematodes, Bacillus thuringiensis
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