Analysis On The Inheritance Of Resistance To Verticillium Wilt Of Upland Cotton And The Diffierentially Expressed Genes After Interacted With Verticillium Dahliae

Cotton Verticillium wilt, a vascular disease caused by Verticillium dahliae Kleb., severely impact the cotton production. In china, upland cotton (Gossypium hirsutum) is the main variety in the production, but this variety is poor resistance to Verticillium wilt. It is difficult to be controlled, so breeded the resistance variety is an effective and economical method to control Verticillium wilt. Studies on the inheritance of resistance to Verticillium wilt of upland cotton, and the identification of differently expressed genes about the interactions between upland cotton and V. dahliae Kleb., would provide significant theoretical foundation for breeding resistant upland cotton varieties. The inheritance of resistance to Verticillium Wilt of G. hirsutum Zhongzhimian KV-3 was studied by crossed between the susceptible G. hirsutum KV9-1 and the resistant G. hirsutum Zhongzhimian KV-3, then the disease resistances of the populations of P1,P2,F1,BC1,BC2 and F2 generations were identified in the V. dahliae nursery. Investigation and statistic of the resistant performance of the six generations were used for analyses on the inheritance of Verticillium wilt resistance of the G. hirsutum Zhongzhimian KV-3. The results indicated that resistance of Zhongzhimian KV-3 to Verticillium wilt might be controlled by a dominant gene and two additive genes, and the additive effect is more important. When the two additive genes simultaneously exist, the individual performs resistance, and when only one additive gene exist, the individual performs tolerance, and when all the two genes don't exist, the individual performs susceptible.In this study, three upland cottons were taken as tested plant materials, obtained two high resistance varieties of Zhongzhimian KV-3 and Ning 4-14 and one high susceptible variety. Then, used the technique of cDNA-AFLP to study differentially expressed genes in interaction of the three upland cottons with the V. dahliae V991 strain. Results showed the total of 83 differentially expressed bands was obtained. Zhongzhimian KV-3 have 29 different bands, and 18 up-regulated, 11 down-regulated; Ning 4-14 have 28 different bands, and 19 up-regulated, 9 down-regulated; KV9-1 have 26 different bands, and 17 up-regulated, 9 down-regulated. The fingerprint in PAGE of the two resistance varieties were similar and 10 different bands are same, but no same different band with the susceptible variety KV9-1.The up-regulated bands of the two resistance varieties were picked out and cloned, 13 TDFs were gained in all, and 6 TDFs are both in the two varieties. Results of blastn identification in NCBI showed there are 9 TDFs have high homology nucleotide sequences to Gossypium, 1 TDF has high homology to Cicer arietinum,and 2 TDFs have no homology nucleotide sequences. Results of blastx identification in NCBI showed there are 11 TDFs have homology protein sequences, obtain DEAD box ATP-dependent RNA helicase, cytochrome P450, exostosin-2, thiamine pyrophosphate enzyme, reverse transcriptase, periplasmic beta-glucosidase precursor, serine C-palmitoyltransferase, class III peroxidase and senescence-associated protein. They are maybe paly an important role to defend the V. dahliae in the upland cotton. 2 TDFs encord hypothetical protein and 2 TDFs have no homology protein sequences, the function of them have to deeply study.