The Study Of The Embryonic Development And Cell Culture Of Odontobutis Potamophila

Embryonic development, snout tissue culture, and the karyotype analysis of Odontobutis potamophila were studied in this paper, which will provide a theoretical basis for their offspring culturing and artificial breeding.The embryo development of Odontobutis potamophila was studied in the present paper. Unfertilized eggs of Odontobutis potamophila were artificially inseminated and incubated at 21±1℃. The progress of embryonic development was observed under light microscope and pictures were taken each hour. The progress of embryogenesis took 502 h since fertilization and could be divided into 8 periods including 35 stages. According to the number and size of blastomeres, formation and epiboly extent of the blastoderm, development of the central nervous system, the numbers and formation of somites and the developmental features of the organs, the whole progress was divided into zygote period, cleavage period, blastula period, gastrula period, neural period, segmentation period, pharyngula period and hatching period. The periods then were divided into different stages. The stages, and their names, are based on morphological features and other significant events. The cleavage subdivided into 2～64 cell stages and morula stage. The blastula period subdivided into early blastula stage, middle blastula stage and late blastula stage. The gastrula period subdivided into early gastrula stage, middle gastrula stage and late gastrula. The neural period included early neural stage and late neural stage. The segmentation period subdivided into 2～41 somite stages. The pharynula period subdivided into digestive system stage, liver-forming stage, branchial cleft stage, spleen forming stage, mouth stage and swim-bladder forming stage. The research of the embryonic development of Odontobutis potamophila provides a theoretical basis for their offspring culturing and artificial breeding.The cells of snout tissues derived from Odontobutis potamophila were studied in vitro with the medium 199. The snout cells had been subcultured to the fourth generation. The snout cells migrated from the tissues after 6～8 h in vitro. After that, cells could be done once ervery 4～6 days. The cells were fiber-like cells in primary culture and subculture. These results could provide a theoretical basis for genetics, virology and toxicological research.The kidney tissue of Odontobutis potamophila was used for the karyotype analysis, with PHA injection and cochine of Linyihao for sample preparation. Our results showed that the karyotype was 2n=44t, NF=44.