Molecular Mapping Of Stripe Rust Resistant Gene In Zhongliang93444 And Genetic Analysis In Xiao Yan6 And Kang Yin655

Stripe rust, caused by Puccinia striiformis f.sp. tritici, is one of the most serious diseases of wheat in china. In epidemic years can cut wheat production from 20 to 30%and even can give up wheat production.Used resistance disease cultivars is a most effect,economic,frfriendly to enriroment way for controlled stripe rust. However, the occurrence new races with added new virulence will led a breakdown of wheat resistance to stripe rust. Geneticists and plant breeders pay more attention to wild relatives and elite cultivars carring durable resistance in order to resolve a breakdown of wheat resistance to stripe rust. The genetic analysis and molecular mapping of genes resistance to stripe rust in Zhongliang9344,Kangyin 655and Xiaoyan6 were investigated. The results as follow:1.To identify new resistance genes to yellow rust,the F2,F3 progeny of the cross of Zhongliang93444/Mingxian 169 were inoculated with the yellow rust race CYR30,CYR31 and CYR32 at the seeding in the greenhouse. The result indicated that Zhongliang93444 has effective resistance at seeding-stage to the dominant races in China, The resistance to CYR30 and CYR31was controlled by one dominant and one recessive gene,respectivly, the resistance to CYR32 was controlled by two complementary dominant genes, while F3.3 resistance to the race CYR32 was controlled by one dominant gene named Yr93444 temporarily.2. F3.3 segragated family from the Zhongliang93444 crossed with susceptible cultivar Mingxian169 were mapping by SSR, eight SSR markers Xgwml22,Xwmc702,Xwmc644,Xwmc794,Xgwm328,Xwmc455,Xgwm372,Xwmc819 are linked to Yr93444 were fined on chromosome 2AL. The genetic distances to Yr93444 was 38.1 cM,30.7cM,22.9cM,15.6cM,10.0cM,6.9cM,3.5cM,2.8cM,respectivly.The pedigree analysis and molecular maker results indicated the origin of a novel gene from Elytrigiaintermedium. Xgwm372,Xwmc819 closed to dlinked Yr93444 as PCR primers were detected in most of zhongliang wheat cultivars and was not detected in 86%of the 42 tested wheat cultivars from Huang-huai area.3. The F2 and F3 progeny from crossed Xiaoyan 6 with susceptible cultivar Mingxianl69 were tested with the current epidemic yellow rust race CYR30,CYR32 and Sull-4 for genetic analysis of resistance to the dominant races in China at adult-plant stage.The'Xiaoyan 6'resistance to race CYR30 and CYR32 was controlled by the resistance complementary action of two pairs of recessive genes. The resistance to the race Su11-4 was controlled by one dominant gene.The results shown that the High-temperature adult-plant (HTAP) stripe rust resistance haved harboured in Xiaoyan 6 and is a very impotant usefull for breeding resistance cultivars to stripe rust.4. The F2 and F3 progeny from the Kangyin655 crossed with susceptible cultivar Mingxian169 were inoculated with the current epidemic yellow rust race CYR29,CYR30 and Su11-14 for genetic ananysis of resistance to stipe rust. The result indicated that Kangyin655 has effective resistance to the dominant races in China at adult-plant stage.the Kangyin655 resistance to the race CYR29 and CYR30 were controlled by the resistance complementary action of one dominant and one recessive gene.The resistance to the race Su11-14 was controlled by two dominant genes.The results suggested that Kangyin655 resistance to tripe ruste in adult-plant has a very impotant value for breeding resistance varieties to contrulled stripe rust preventiont.