Effects Of The Secondary Metabolites Of Rhizotonia Leguminicola On Immune Function Of Immunosuppressed Mice

Objectives:This trial was to study the effects of the secondary metabolites of Rhizotonia leguminicola on cyclophosphamide (CTX)-induced immunoregulation of immunosuppressed mice. The immunosuppression model was established by CTX injected into abdominal cavity of the KunMing Mus musculus albus. The number of PWBC, index of organ, phagocytic activity of peritoneal macrophage, hemolysin level in peripheral blood, lymphocyte transformation of spleen, activity of NK cells were measured and pathology changes were observed to explore the effect of immune function of mice on both union application. All these could provide rationale for the secondary metabolites in clinical application.Methods:①The mice were injected intraperitoneally with 80 mg/kg CTX everyday, three times consecutive, to establish the animal model which could result in immunosuppression.②The mice were infused CTX and the secondary metabolites of Rhizoctonia leguminicola which had swainsonine respectively at 8,16,32 mg/kg. The secondary metabolites of Rhizoctonia leguminicola and CTX were jointly utilized and were divided into 5 groups randomly: groupⅠ(normal saline), groupⅡ(CTX 100 mg/kg), groupⅢ(SW8 mg/kg + CTX 80 mg/kg), groupⅣ(SW16 mg/kg + CTX 80 mg/kg), groupⅤ(SW32 mg/kg + CTX 80 mg/kg); 24h after the last administration, The number of PWBC, index of organ, phagocytic activity of peritoneal macrophage, hemolysin level in peripheral blood, lymphocyte transformation of spleen, activity of NK cells were measured. This trial was to explore the effects of secondary metabolites of Rhizotonia leguminicola on the immune function of CTX-induced immunosuppressive mice and the possible mechanism, and to obtain the safeguard dose. In the end, animals were dissected to observe the histopathology in liver and kidney.Results: CTX was injected consecutively into the abdominal cavity at 80 mg/kg for 3d, which leaded to immunosuppression for the mice. The groups which contained SW at 8 and 16 mg/ (kg·d) could increase the number of PWBC of the mice. The CTX induced a decrease in phagocytic activity of peritoneal macrophage of mice and a boost of hemolysin level in peripheral blood, lymphocyte transformation of spleen and the activity of NK cells, which is especially workable for the group contained SW at 16 mg/(kg·d). Conclusion:①CTX was injected consecutively into the abdominal cavity at 80 mg/kg for 3d, which leaded to immunosuppression for the mice.②The secondary metabolites of Rhizotonia leguminicola (the dose of SW at 8,16 mg/kg) has effects on the recovery and reinforcement of CTX-induced immunosuppression.