Copmarative Proteomic Analysis Of The Lymphoid Organ In Fenneropenaeus Chinensis Response To Inactivated Vibrio Anguillarum Stimulation

The sustainable development of shrimp aquaculture industry is facing serious challenges including shrimp diseases and deterioration of environment in the world. The frequent occurrence of shrimp diseases caused by bacteria and virus has led to serious economic loss of shrimp farming production. The environmental stress caused by the deterioration of aquaculture environment not only stunts the normal growth of shrimp, but also lead to the decrease of immune capability which could induce an increased susceptibility to infectious disease of shrimp. The studies on changes of proteins expression patterns in shrimp challenged by pathogen infection are very important for us to know more about the mechanisms of anti-disease in shrimp, and provide more knowledge about anti-disease shrimp culture.As an important immune-related organ, the lymphoid organ in penaeid shrimp is a specific site to eliminate invading pathogens. The study of shrimp lymphoid organ is an important part for understanding the immune mechanism in penaeid shrimp. To gain an insight into the function of shrimp lymphoid organ at protein level, we analyzed the proteome of lymphoid organ in healthy Chinese shrimp Fenneropenaeus chinensis (F. chinensis) through two-dimensional gel electrophoresis (2-DE) based on the proteomic approach. We established a high-resolution, reproducible 2-DE reference map of lymphoid organs in Chinese shrimp, in which a total of 95 spots representing 75 protein entries were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) with both online and in-house database. According to Gene Ontology (GO) annotation of biological process, the identified proteins were classified into 13 categories. Among them, approximately 36% of proteins related to cytoskeleton are noticeable. On the basis of the reference map of Chinese shrimp lymphoid organ proteome, a comparative proteomic approach was employed to investigate the differentially expressed proteins in lymphoid organ of Vibrio anguillarum-challenged F. chinensis. At 24 h post-injection (hpi), 17 differentially expressed protein spots were successfully identified, including 4 up-regulated protein spots (represent 4 proteins: cathepsin L, protein similar to squid CG16901-PC, protein kinase C and protein similar to T-complex Chaperonin 5 CG8439-PA), and 13 down-regulated protein spots (represent 9 proteins: actin, beta-actin, cytoplasmic actin CyII, alpha tubulin, beta tubulin, protein similar to proteasome delta, vacuolar ATP synthase subunit B, elongation factor 2, carboxypeptidase B). These data may help us to further understand the function of lymphoid organ and the molecular immune mechanism of shrimp responsive to pathogen infection.