Preparation And Effect Of Controled-release Butyrate On Intestinal Barrier In Early-weaned Piglets

Although early weaning increases the breeding sows utilization, weaning stress causes seriously adverse effects on piglet growth, resulting in "Early Weaned syndrome" and huge economic losses to the livestock Industry. And this result is mainly due to severely damaged intestinal barrier by weaning stress in piglets.It is proved that butyrate is a potent growth inhibitor to cancer cells. Moreover, butyrate lower cholesterol absorption; modulates intestinal microbe flora; severs as primary energy source for normal colonic epithelium; decreases luminal pH, which results to improved activity of certain enzymes; plays an important role in modulating colonic electrolyte transport and stimulating sodium and water absorption; and stimulates secretion of intestinal mucins. In this study, a kind of controlled-release butyrate material will be developed and the effect of controlled-release butyrate on intestinal barrier function will be evaluated in early weaning of piglets.120 piglets weaned at 28 d of age were randomly divided into 4 groups of 3 replicates each (10 piglets per replicate) by ancestry, weight and sex, which were fed basal diet (control), basal diet+1 mg/kg sodium butyrate (testⅠ), basal diet+1 mg/kg controlled-release butyrate (testⅡ) and basal diet+2 mg/kg controlled-release butyrate (testⅢ). Piglets were allowed ad libitum access to creep feed and water. The piglets (2 piglets per replicate) were bled from the anterior vena cava with heparinized vacutainer tubes at 8:00 at 56 day of slaughter. The sample of intestine, plasma, lymph nodes were taked and preserved until analysis.The results showed:The specific surface area of carrier silica gel is 297.34 m2/g and average pore size,85.26A. The saturated vapor pressure of controlled release butyric acid is 0.03kPa at the room temperature of 25℃. TG/DTA analysis showed that the content of butyric is 37.5% in controlled-release butyric material.Compared to control group, final weight in test group was increased by 2.86% (P>0.05),3.57% (P>0.05) and 5.51% (P>0.05), and there was no significant diffrence in the test group (P>0.05); average daily gain of test group was respectively increased by 4.77%(P<0.05),6.03%(P<0.05) and 9.29%(P<0.05), and there was significant diffrence between testⅢand testⅠ; There was no significant diffrence in average daily intake and F/G among all treatments. Diarrhoea rate in the test group respectively was decreased by 47.33%(P<0.05),50.17%(P<0.05) and 58.15% (P<0.05) compared to the control group.Compared to control group, serum cortisol in test group was respectively decreased by 37.06%(P<0.05),44.00%(P<0.05) and 52.7%(P<0.05). And there was no significant diffrence in test group (P>0.05).Plasma D-lactate level in test group was respectively lower than control group by 47.36%(P<0.05),52.64%(P<0.05) and 67.72%(P<0.05). Compared to control group, endotoxin in the test group was respectively decreased by 31.12%(P<0.05),41.07% (P<0.05) and 86.98%(P<0.05), and there was significant diffrence between testⅢand testⅠ,Ⅱ. DAO in test group was significantly decreased by 50.11%(P<0.05), 55.21%(P<0.05) and 64.97%(P<0.05), and there was no significant diffrence among test group (P>0.05).Compared to control group, the number of Jejunal E coli. were significantly decreased by 3.79%(P>0.05),12.48%(P<0.05) and 16.90%(P<0.05), respectively in test group. The number of appendix E coli. in the test group were decreased by 5.12% (P<0.05),11.83%(P<0.05) and 14.72%(P<0.05) compared to control group. The number of colon E coli. were respectively lower than control group by 4.22% (P<0.05),11.66%(P<0.05) and 14.58%(P<0.05).Bacterial translocation rates of mesenteric lymph nodes in control group was 83.33%, however, testⅠfell to 33.33%, testⅡdecreased to 16.67%, and there was no bacterial translocation rates of mesenteric lymph nodes in testⅢ.Compared to control group, GSH level in test group was respectively increased by 10.20%(P>0.05),15.55%(P>0.05) and 32.03%(P<0.05), and there was no significant diffrence among test group (P>0.05). SOD level was higher than control group by 13.12%(P>0.05),19.20%(P>0.05) and 36.41%(P<0.05), respectively in test group. MDA level of tesf group was significantly decreased by 62.23%(P<0.05), 72.73%(P<0.05) and 87.41%(P<0.05) compared to control group. Compared to control group, villus height increased, crypt depth decreased and mucosal thickness decreased in test group. There was no significant difference in jejunal villus height between testⅡand testⅢ(P>0.05), but jejunal villus height of testⅡand testⅢwas significant higher than control group and testⅠ(P<0.05). Crypt depth of testⅢwas significant lower than other treatment groups (P<0.05). There was no significant difference in villus height/crypt depth and mucosal thickness among all treatments (P>0.05). Ileum villus height of control group is lower than testⅠand testⅡ(P>0.05), but there was significant difference between control group and testⅢ. There was no significant difference in crypt depth, villus height/crypt depth and mucosal thickness among all treatments (P>0.05).The result showed that the early-weaning piglets was in stress and intestinal barrier funcetion was destroyed. Controlled-release butyrate could ease weaning stress, decreased intestinal permeability, restore intestinal microflora balance, repare intestinal mucosal, increased intestinal antioxidant, thereby reducing the effect of the stress of early-weaning.