Cloning Of SVP-like MADS-box,MADS12 Gene From Grape And Construction Of Plant Expressing Vector

Grape is one of the most important fruit trees in the world, its culture area and total yields are advanced in the world. it is a great significance to study the mechanism of flower development on fruit trees cultivation, breeding and promoting the development of fruit production.A SHORT VEGETATIVE PHASE (SVP) homologue was isolated from Vitis vinifera x V. labrusca'Fujiminori'inflorescence by bioformatic analysis and RT-PCR. It is 684 bp, coding a polypeptide of 227 amino acids, named as VvSVP. Homology analysis showed that the deduced VvSVP protein was highly homologous to the MADS-box genes, PMADS1, MdJOINTLESS,PkMADS,SVP, the identity are 84.1%,81.7%,78.9% and74.9%, respectively. Phylogenetic analysis also indicated that VvSVP belongs to the STMADS11 lineage in MADS-box gene family. Expression pattern analysis showed that VvSVP expressed in buds, leaves, inflorescenes, fruits, but not expressed in flowers, pedicels, stems and roots. Blast of VvSVP with a shotgun genome sequence indicated that VvSVP genome is consist of 8 exons and 7 introns, the number and length of exon is not the same as SVP.Electronic VvMADS12 homologue was isolated from Vitis vinifera×V. labrusca 'Fujiminori'inflorescence by bioformatic analysis and RT-PCR. It is 630 bp, coding a polypeptide of 209 amino acids, named as VvMADS12. Homology analysis showed that the deduced VvMADS12 protein was highly homologous to the MADS-box genes, Pd MADS-box,AmDEFH7,MODEFH7,SlTDR8,CsMADS-box,PrMADS-box,ZmMADS15the identity are 70%,63%,62%,60%,66%,45%,and50%, respectively. Phylogenetic analysis also indicated that VvMADS12 belongs to the TM3 lineage in MADS-box gene family. Expression pattern analysis showed that VvMADS12 expressed in buds, leaves, inflorescenes, fruits, but not expressed in flowers, pedicels, stems and roots. Blast of VvMADS12 with a shotgun genome sequence indicated that VvMADS12 genome is consist of 8 exons and 7 introns.We constructed plant overexpression vector of VvSVP and VvMADS12.