The Study On Pretreatment And Lignocellulose Decomposability To Cotton Straw In Xinjiang

The exhausted fossil energy source be substituted by the ethanol that is made from cellulose have amazing future. The physical chemic and biologic pretreatment based on the cotton straw in xinjiang are researched in this text, we expect for finding a clean and inexpensive way that can change the cellulose into glucose efficiently.Different cellulose differential medium are compared in this text, and a differential medium that can filtrate cellulose decomposing microorganisms efficiently by dint of experimentation is gained. Cellulose and lignin decomposing microorganisms are gained throμgh the first filtration and the second filtration. Different physical chemic pretreatments are compared, and the best pretreatment that bring on the best activity of cellulase is gained. Cotton straw's synergistic decomposing with cellulose and lignin decomposition microorganisms is studied in the text, and the best biologic pretreatment that bring on the best activity of cellulose is gained. The best physical chemic and biologic pretreatment are compared in the text.The best cogon red differential medium's component is confirmed in experimentation, they are CMC-Na MgSO₄ K₂HPO₄ cogon red agar glutin and the steep of soil. Six cellulose decomposing microorganisms with high sporule yield and five lignin decomposing microorganisms (white rot fungi) are obtain, the white rot fungi F11 have the best decomposability on lignin. Two cellulose decomposing microbes are gained, which can commix with F11 to cultivate. The best physical chemic pretreatment condition is that the cotton straw after ultrasonic treatment with alkali as C source, beef extract as N source, 40℃as ferment temperature and 7 days as ferment time. The first influence factor to the activity of enzyme is temperature, and the second is N source. When F52 commixed with F11 to cultivate, CMCase and FPAse both increased, Lac disappeared, MnP decreased and LiP increased, the yield of glucose increased. Compared to the top of CMCase after physical chemic pretreatment, the top of CMCase after biologic pretreatment is lower.