| In the veterinary clinic, pigs, sheep, cattle, horses, dogs and other animals, the clinical di gestive diseases accounted for about 30% of the total amount of disease, and loss of appetit e, indigestion, constipation, bloating, Ruminis, flaccid and other gastrointestinal symptoms, al most the majority of diseases in animals with or secondary. For the treatment of these symp toms, often used carbachol, neostigmine and other western medicine to be cholinergic, but to xic, so strong. Traditional Chinese Veterinary Medicine on the treatment of gastrointestinal d ysfunction has extensive experience in veterinary medicine and the rich source of side effect s and use in veterinary treatment of gastrointestinal disorders has been increasing attention. C hina to vigorously promote and promote the modernization of Chinese medicine, one of the important elements is the "process of traditional Chinese medicine preparation, in the traditio nal soup, powder, balls formulations based on the principles of Western medicine by the add ition of new formulations."To this end, we choose from"Chinese Veterinary Pharmacopoeia" (2005 version 2) used in the primaries, the stomach class veterinary drug powder, to the corresponding granules w as prepared using rabbit intestinal tests, from 8 granules relative to the role of screening effi cacy of the best of a powder, granules will be screened for further processes, efficacy, toxic ity studies. This article is divided into five parts:First part of the stomach class veterinary formulation. In this study, "Chinese Veterinary Pharmacopoeia" (2005 version 2) 8 used in the primaries, the stomach class veterinary drug powder, to the corresponding granules was prepared using rabbit intestinal tests, from 8 Gra nules in the role of screening efficacy relative to the best of a powder.. The results showed that with gentian, Atractylodes, Bupleurum, Ginger, and the composition of the pig health so dium bicarbonate powder particles were prepared by (tentatively named Pig Health particles, the same below), can significantly increase the intestinal contractile tension, the drug health effect is better than pigs loose. Therefore, this article selected healthy pigs particles for furt her process, efficacy and toxicity studies.The second part of the pig health granular optimization of single Chinese herb extracti on and preparation of granules. (1) gentian extract active ingredients to ethanol concentration, extraction temperature, solvent usage and extraction times investigated factors to yield genti opicroside index. The results showed that the optimum extraction conditions were:70% etha nol concentration, extraction temperature 80℃,10 times the amount of solvent extraction fo r 3 times; 2) the dark green technique effective component's extraction, take soaks the time (h), the watering quantity (time) and the extraction time (h) as the inspection factor,and take s the target by the dark green technique volatile oil's catch basin, finally the dark green tec hnique volatile oil's optimum extraction condition is:Immersion time 12h, the watering meas ures 10 times, the extraction time is 8h; (3) the extraction of effective components Bupleuru m to soaking time (h), add water (times) and extraction time (h) to examine factors in orde r to collect the amount of volatile oil Bupleurum as an indicator, the results Bupleurum opti mum oil extraction conditions:immersion time 12h,10 times the amount of water, extractio n time is 5h.Pig health Nanoparticles:take Ginger 10g,20g of sodium bicarbonate and extracted the gent ian, herb, and Bupleurum residues, followed by adding eight times the amount of 6 times,4 times the amount of water boiling 2h. Filter, collecting the filtrate, merger filtrate, filtrate a nd the herb,. Bupleurum combined aqueous solution after distillation, filtration. Heating the filtrate concentrated to about 500ml, natural cooling to room temperature. Volume of filtrate was added 40%,60%,80% ethanol, mix well, put it aside for 24h precipitation temperatur e. Filtration, the filtrate collected, will be made of pig health prescription drugs relative bul k density 1.62 of liquid extract. By liquid extract:starch:sucrose (flour)= 1:2:0.5 ratio, mi xing, manufacturing of soft material; Been made from wet-sieve particle 2; Drying the wet granules at 55℃for 18h, were dry particles (water content 2.3%); The dry granules were s ieve over 1,5 Intact; Essential oil, volatile oil Bupleurum dissolved in ethanol (volatile oil:e thanol= 1:3), even after the spray particles in the whole grain surface, moderate mixing, dr y or too.Optimization of particles before and after the pig health efficacy validation:Test by isolated rabbit intestine, before and after the process optimization efficacy particles of pig health cert ification. Found that at the same concentration, optimization of the ratio of particles is not o ptimal efficacy pig health is better.The third part is pig health Pharmacological tests. (1) small intestine test:Powder each group only 6.67g/kg,0.2mL/10g (body weight) administered orally, each granule group only 6.37g/kg,0.2mL/10g (body weight) administered orally for 5 days, ban Fresh water can not help but 12h, 1h in section 6 after administration, each mouse fed with carbon powder susp ension,0.2mL,20min after injection. Remove the entire gastrointestinal tract, pyloric to the i leocecal measuring length and pyloric to the carbon front end of the distance, calculate the percentage of carbon powder to promote. The results showed that particles pig health gastroi ntestinal motility in mice was superior to casual pig health. (2) gastric emptying test:each p owder group only 6.67g/kg,0.2mL/10g (body weight) administered orally, each granule grou p only 6.37g/kg,0.2mL/10g (body weight) was administered orally administration, for 5 day s. Fasting can not help but water 12h. 1h in section 6 after administration, each mouse fed 0.1% methyl orange solution 0.2mL.20min after injection. Gastric contents obtained with 10 mL distilled water and diluted with 5% NaHCO3 pH adjusted to 6.0～6.5 to 2000r/min Ce ntrifuge 10min, the supernatant was measured at 420nm wavelength of the optical density of methyl orange.0.1% methyl orange solution after mixing 0.2mL by adding lOmL of distill ed water as the base for measuring the optical density optical density of methyl orange. Cal culated gastric residual rate of methyl orange. The results showed that the pig gastric emptyi ng in healthy mice, the role of particles on health is better than pigs loose.Part IV is Part particles pig health safety trials. (1) Pig Health particles acute toxicity t est:Through pre-test, the mice fed a maximum concentration of particles healthy pigs (7.06g /mL) and mice fed the maximum size affordable (0.4mL/10g). Ig 14d, the mice all survive d. Therefore, the particles can not be measured LD50, suggesting that the particles are relati vely safe. Further determination of the particles on the maximum tolerated dose in mice:Te st group dose per mice 0.4mL/10g pigs fed 7.06/mL Health particle concentration, the contro 1 group was given equal volume of distilled water, were administered three times at intervals of 6h. The results showed that, in the 14d consecutive observation, mice did not appear co nvulsions, tremors and other abnormal behavior, breathing, eating normal, freedom of movem ent, no death after administration of 14d. Pig Health particle maximum dose in mice was 0. 04mL/g×7.06g/mL×3×1000= 847.2g/kg, the equivalent of Veterinary Pharmacopoeia provides the maximum clinical dose scattered pig health to 127 times the amount of 6.67g/ kg. (2) particles pig health long-term toxicity test:Pig Health particles located high dose gr oup (266.80g/kg, equivalent to 40 times the recommended clinical dosage), middle dose grou p (133.40g/kg, equivalent to 20 times the recommended clinical dose), low dose group (66.7 Og/kg, equivalent to 10 times the recommended clinical dosage), administered volume 10.0 mL/kg weight in the control group to equal volume of distilled water for consecutive 60d. T he results showed that experimental group in general examination, blood parameters and orga n coefficient in each group compared with the control group, there was no significant differe nce (P> 0.05), showed no long-term toxicity of particles pig health.The fifth part is on quality standards for pig health Study. (1) General examination:Th e appearance of particles of pig health, size, moisture content, melting of an inspection are i n line with requirements. (2) Determination:HPLC detection of porcine established health G entiopicroside particles, method of content atractylenoideⅢ, made 5 batches of swine health particle samples. The results show that particles containing pig health 2.14～2.72% of gen tiopicroside, with 0.02～1.01% of atractylenolideⅢ; chemical determination of five batches of samples in the sodium bicarbonate content of 11.37～12.94%. (3) Identification:Using thin layer chromatography on pig health granules Bupleurum, Ginger had identification.
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