SNPs Identification Of PRLR Gene And Associations With Egg Production In Goose

PRL which is secreted from anterior pituitary gland has extremely a broad spectrum on biological role.PRL mainly relates to reproduction in avian species.Increased concentration of Prolactin plays an important role in the cessation of egg laying and broodiness during the active laying period.Without exception,the prolactin acceptor adjusts these effects.Many researches show that physiological action of Prolactin(PRL) is that the prolactin acceptor(PRLR) is upright by being located in the upper target cytomembrane prolactin lead.Stubborn broodiness of local avian species mainly attributes to their low egg yield.PRLR has been cloned in recent years in chicken,turkey,pigeon etc. The feather is filled with nature and the prolactin acceptor goes hand in hand.That our country's local bird has strong broodiness is one major factor of low egg laying.In general, PRLR gene which relates to broodiness was selected as candidate one.By genome comparison,10 pairs of primers was designed using Primer Premier5.0 and DnaStar programme in cloning the whole PRLR coding region of Wanxi-White goose.PCR-SSCP method along with fragment sequencing was used on testing gene mutation at exon 4,6,10 and 5'UTR site.Correlation between mutation in PRLRgene and egg production was analyzed.Meanwhile,sequence of PRLRcoding region and mutation detection at gene coding was also analyzed on Rhine goose to compare with Wanxi-White one.The complete PRLR coding region 5'UTR of Wanxi-White goose and Rhine goose was cloned(GenBank accession number:EU534407) by the method of Genomic Comparison.Homologic analysis suggested that the homology of Wanxi-White goose PRLR coding region was 88%and 94%with chicken and duck,PRL Rcoding region respectively.Polymorphisms of exon 4,6,10 and 5'UTRon Wanxi-White geese were analyzed. The results showed that,no polymorphic site was detected at exon 4 and 6 amplified fragment;exon 10,whose overall length expands more than 1,200 bp,so having designed that 6 a pair of primers,The overall length expands 5' UTR of clone to 455 bp,having designed that 2 a pair of primers increasing a fragment.2 SNPs were detected at exon PRLR10-2 and PRLR10-3 fragment amplified.by PRLR10-2 and PRLR10-3 prime respectively.A181G mutation in Wanxi-White goose PRLR gene resulted from the change of Met64Leu amino acid,while-/22A was a synonymous mutation.Genetic analysis suggested that,among Wanxi-White goose populationm,two allele frequencies detected by PRLR10-2 primer were 0.7056 and 0.2944 respectively,allele frequencies detected by PRLR10-3 were 0.8389 and 0.1611 respectively.In Wanxi-White geese population about egg yield records,three genotype frequency at exon10-2were 0.633,0.145 and 0.222 was corresponding to egg yield of 21.46±6.20, 22.21±5.95 and 28.97±5.74 respectively.Least square analysis showed that genotypes detected by 10-2 primer had significant relations with egg yield.Further research is needed on whether polymorphism detected at exon PRLR10-3 has relation with egg production.