The Mutation Research For Mutant Of Rice Variations Nipponbare And "9311"

Rice(Oyrza sativa L.)has become a model plant for the study of the cereal genomes in the last decade.Recent completion of the draft sequence for the rice genome has resulted in an explosion of information on rice genes.The challenge for the post-sequencing era is to identify the biological functions for these genes.Of all the approaches used for discovering the gene function,the more useful one is based on a saturated mutagenesis population,which is a straight and efficient way to understand the role of all the genes.In all the methods for constructing the mutant liberary,the physicaland chemical mutangenesis might be the main methods,which were simple and effective.They can be used to produce a large number of mutants with high mutagenesis frequencein a short period and to establish a mutant population.The purpose of the present experiment is to screen different plant morphogenesis mutants and rice quality mutants by treating the seeds of the Nipponbare and indica rice variety 9311 with ion beam andγ-rays,of which has been completely sequenced in the whole genome.The mutants created by ion beam implantation andγ-rays irradiation will be helpful for gene identification and gene functional analysis.Main results are as follows:1.The seeds of Nipponbare and "9311" were treated with ion beam andγ-rays. Some mutants with the variations of leaf,stalk,panicle or root traits were found in the progenies.In the M₂ generation,740 and 571 mutants were induced by ion beam implantation in "Nipponbare" and "9311",which the rate of different dose between with 1.35%～2.15%and 0.97%～1.89%,the average mutation frequency were 1.64%and 1.27%,respectively;the other 661 and 781 mutants byγ-rays irradiation,which the rate of different dose between with 0.47%～2.13%and 0.51%～3.15%,the average mutation frequency were1.48%and 1.74%,respectively.The mutants created by ion beam implantation andγ-rays irradiation will be helpful for gene identification and gene functional analysis.Compares with theγ-rays mutagenesis,in not only the nitrogen ion beam mutagenesis community has withγ-rays mutagenesis community in the same mutant,and has also discoved some new mutants without inγ-rays mutagenesis.2.The sensitivity of screening with GA and PP333 showed that in the GA₃ screening test,Indica rice has more sensitive than Japonica rice and the tall has more sensitive than dwarf,the average height of Inddica rice and Japonica rice increased 67.18%and 54.92%,the tall and dwarf is 61.97%and 57.44%;in the PP333 screening test,Japonica rice has more sensitive than Indica rice and there is no obviously different with the tall and dwarf,the average height of Inddica rice and Japonica rice increased 155.4%and 193.0%,the tall and dwarf is 152.2%and 149.2%.3.The total 89 plant height mutant lines induced from rice cultivars Nipponbare and "9311" by ion beam implantation andγ-rays irradiation were used for analysis of correlation between plant height and yield component.The results indicated spike length,ear grains,thousand-grain weight,stem dry weight and yield have significantly positive correlation with the plant height,while the number of productive ear has significant negative correlation with it.The correlation analysis of the plant height and yield component will help to reveal the influence of the plant height and the factors of yield component.