Study On Regeneration System Of Section Aigeiros Clone SN05-11 And LN05-51

Section Aigeiros is the most important section of Populus in Hubei Province,and it has been most widely cultivated and plays significant economy role on forest production.Populus deltiodes 'Lux' and P.×euramericana 'San Martino' are respectively the superior clone of P.deltoides and P.×euramericana.They were introduced from Italy in the 1970s and have been widely cultivated in Hubei Province. P.liaoningensis is the superior clone of Section Aigeiros.It was introduced from forest science institute of Liaoning Province in the 1999s and grew well in Hubei Province. SN05-11 is the progeny of P.×euramericana 'SanMartino'×P.liaoningensis and LN05-51 is the progeny of P.deltiodes 'Lux'×P.liaoningensis.They have significant heterosis in growth.In this paper,SN05-11 and LN05-51 were used as experimental materiais.Factors that affecting plantlet regeneration,including different explants,plant growth regulator, the pH value,argar concentration and sucrose concentration,were investigated in details.The major results were following:1.Leaves and petioles were the best explants of SN05-11 and LN05-51.The optimal sterilizing methods of petioles were dipping to 75%Ethanol for 30sec,and then treating with 0.1%HgCI₂ for 1min,the survival rate of SN05-11 was 81.1%,the survival rate of LN05-51 was 86.7%.The optimal sterilizing methods of leaves were dipping to 75%Ethanol for 30sec,and then treating with 0.1%HgCI₂ for 3min,the survival rate of SN05-11 was 80.0%,the survival rate of LN05-51 was 83.3%.2.The optimal buds induction medium of SN05-11 is MS+6-BA 0.5mg/L+NAA 0.05mg/L and the induction percentage is 78.4%.The optimum buds induction medium of LN05-51 is MS+6-BA 0.5mg/L+NAA 0.1mg/L+TDZ 0.02mg/L and the induction percentage is 81.6%.3.The most optimal proliferation medium of SN05-11 was MS+6-BA 0.1mg/L+ NAA 0.01mg/L and the proliferation coefficient was 5.0.The most optimal proliferation medium of LN05-51 was MS+6-BA 0.1mg/L+NAA 0.05mg/L and the proliferation coefficient was 5.2.The optimum culture medium for proliferation contained 6g/L argar,30g/L sucrose,pH 6.2. 4.In vitor plantlets displayed characteristics of short and small after proliferation culture.It was necessary to mature shoots for easier rooting.The optimum mature shoots medium of SN05-11 and LN05-51 is MS+6-BA 0.2mg/L+NAA 0.01mg/L.5.The optimum rooting medium of SN05-11 is 1/2MS+IBA 0.2mg/L and the rooting percentage is 94.7%.The optimum rooting medium Of LN05-51 is 1/2MS+ IBA 0.4mg/L and the rooting percentage is 100%.6.The appropariate substance for in vitro plantlets transplantation is sand and soil in 1:1,the survival rate of SN05-11 was 83.5%and the survival rate of LN05-51 reached 93.2%.