Analysis Of Epigenetic Variations Of Different Generations Of Raphanus Sativus×Brassica Alboglabra Allopolyploid

Distant hybridization of plants can cause a wide range of apparent genetic variability, such as: gene silencing, nucleolar dominance, transposon activation, DNA methylation variations and so on, and DNA methylation , in particular cytosine methylation is one of the main epigenetic control mechanisms. Although researches in this area have been reported, but DNA methylation in the hybrid variations of inter-generational studys are rare. In this paper, methylation variations of artificial F₄ and F₁₀ generations of Raphanus sativus×Brassica alboglabra allopolyploid were examined with MSAP, and methylation inhibitors, respectively; In particular F₄, F₁₀ after 5-azacytidine treatment detected by different generations of adversity stress resistance to adversity; Finally, we detected structures of nuclears of F₄, F₁₀ with 5-AC treatment and untreated with electron microscopy to detect impacts of DNA methylation variations for nucleolar structure. The main conclusions were as follows:1. Overall levels of methylation of F₄, F₁₀ generations of Raphanus sativus×Brassica alboglabra allopolyploid were stability, two modles of variations of methylation in both were the main modes of variability , based on DNA methylation in generational transmission of distant hybridization of genetic variation is the existence of epigenetic remodeling.2. After methylation inhibitor 5 - azacytidine treatment, both the overall level of methylation changes in the variability or the extent of methylation by the impact of size, F₄ affected significantly larger than the impact on behalf of the F₁₀, This may be related to the maintenance of the level of genome methylation in the regulation and control mechanisms are not yet ripe for an era of instability, so the impact of adversity are significantly higher than the impact on the stability from generation to generation.3. Observed the nucleolar structures of the longer processing time F₄, F₁₀ of 5 - azacytidine processing, nucleolus configuration change more and more obvious, nucleolar heterochromatin region in a significant decrease in nucleolar in the gradual disappearance of the various structures on DNA methylation in the regulation of nucleolar structure played a key role in the regulation and control.