| Boer goat population were analyzed to look for markers of genes related with growth trait by using methods Of Microsatellite,PCR-RFLP.The specific result as follow:1. microsatellite markers which include MCM218,OAKFCB005,MAF050,MCM064,ETH225,HEL04 and LSCV11 were chosen to analyze the genetic polymorphism of 150 of Boer goat. Result shows 178/178 of MCM218,181/181 of MAF050,202/202 and 220/198 Of OARFCB005,157/167 of ETH225 and 193/167 of MCM064 were positively correlated to growth trait; 198/172 of MCM218,163/163 of MAF050,224bp of OARFCB005,195/195 of MCM064,159/159 and 135/135 of HEL04 had negative correlation with birth trait; 178/178 of MCM218, 153/183 of ETH225 were positively correlated to weaning trait; 198/172 of MCM218, 157/160 of ETH225,194/194 of OARFCB005 had negative correlation with wean trait; 187/167 of MAF050 were positively correlated to one-year-old trait; 171/153 of LSCV11,198/172 of MCM218 had negative correlation with one-year-old weight,202/178 of MCM218 had negative correlation with one-year-old length.2.Carried on the fixed effect of 3 microsatellite were merged,the correlation between merge genotype and the growth trait in boer goat showed the least squares means had reached significant(P<0.05).3. Mutations in PRLR exon 10 only found in Matou goat, Zazhong Matou and Boer by PCR-RFLP, Nubi goat were not found.4.Gene frequency, polymorphism information content, number of effective alleles and heterozygosity were studied in 348 Boer, Matou and Nubi goats using 3 microsatellite markers(LSCV11,MCM218andMAF050). The results indicated that there were genetic polymorphisms at these 3 microsatellite markers which can be used for genetic diversity evaluation in goat breeds. The population genetic variability of Matou goat was the highest, followed by Nubi goat, Boer goat. |
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