Study On The Technical System Of Tissue Culture And Rapid Propagation Of Jacaranda Mimosifolia

The study was mainly to probe an efficient way through tissue and rapid propagation of Jacaranda mimosifolia.The stems with shoot,the leaves,the leafaxles of young branches are taken as explants..For know the better medium and proceeding of culturing Jacaranda mimosifolia.,we use orthogonal experimental design and analysis of variance to establish the technique system of tissue culture.The affected factors of propagation, existing problems in this technique and the corresponding solutions are also described, which provide the theoretical base and technical measures for industrial producing of plantlet.The study of tissue culture technique and the better medium for various stages are as follows:â‘ Initial medium of promoting seed germination was 40～45℃warm water soaking, germination rate was 55.7%;pollution was the lowest for 8.2%.when sterilization with 0.1%HgCl₂ 8minâ‘¡April is the best season to draw materials,in which the germination rate are 47.3 %and 25.6%by stem and leaf,the contaminated rate only are 32.7%and 30.8%by stem, and leafâ‘¢Initial medium of stem:MS+6-BA2.0 mg.L^-1+NAA0.1 mg.L^-1+2,4-D 0.1 mg.L^-1in which the germination rate is 92.3%,and the shoot forming index is 1.5;Initial medium of hypocotyls:MS+6-BA0.5 mg.L^-1+NAA1.0 mg.L^-1+2,4-D1.0 mg.L^-1,in which the callus induction rate is 73.7%â‘£Proliferating of stems cultivation:The optional proliferating buds culture medium stems and shoots wes:MS+6-BA0.5 mg·L^-1+NAA0.5 mg·L^-1+IBA0.5 mg·L^-1,in which the multiplication rate is 3.7and the multiplication of efficiency seedings is 3.5.Highly proliferating callus was obtained following cultivation on MS+6-BA1.0 mg.L^-1+NAA0.5 mg.L^-1+ZT 3.0 mg.L^-1.The proliferating rates could be up to4.2.The best culture medium for callus of stems differentiating was MS+6-BA1.0 mg.L^-1+NAA0.5 mg.L^-1+2,4-D 0.5 mg.L^-1.The regeneration rate was 66.1%.⑤Efficiency seeding culture:1/2MS+IBA0.05 mg.L^-1,in which the rate of efficiency seedings is 85.7%.â‘¥Rooting culture:1/2MS+suger20 g.L^-1+NAA 0.1 mg.L^-1+AC2.0 g.L^-1 in which the rate of rooting is 78.3%,the quantity of roots is 3.4,and the length is 4.2 cm.⑦Forging seeding transplanting:The transplant medium in vermiculite,perlite and riversand by 1:1:2 is the optimal for.Jacaranda mimosifolia,which the rate of survival is 63.6%.