Study Of Terpene Component And Bioactivity Of Propolis

The article focused on the study of terpene component(extraction and separation, component identification,content detection)and bioactivity of propolis. The volatile oil was extracted through vapour distillition. The GC-MS was used to analyze the volatile oil, 54 kinds of constituents were identified; most of them are monterpene and semiterpene. The optimized extraction condition: the ratio between material and water is 1:5;marinating time 0.5 h,refluence time 20 min,distillation time 4.0 h.Refluence method was adopted to extract the effective components. Used chloroform and ethyl acetate as the elution solvent to collect the terpene components. The chloroform part, which has good antibacterial activity, was studied on separation and purification. TLC method was adopted to select condition and column chromatogram method was adopted to separate. Active terpene compound dehydroabietic acid was gained, which content in the pinecone is 0.373%by HPLC detection. Detection of terpene content was studied by Spectrophotometry. Standard equation is A=0.0306C+0.00308, R=0.9998; linearity range is 4.0～32.0 mg/L. Precision and repeatability of the method is good.Bacteriostasis of extraction compounds was proved by paper strip method. The rusults were as follows: Extraction dissolved in 95% ethanol , chloroform extraction and ethyl acetate extraction showed bacteriostasis, whereas, 95% ethanol showed more effectivity. It can be confered that 95% ethanol extraction may have more active extraction. Inhibition of the three extraction solution was enhanced with concentration increased. Minimal inhibitory concentration offers an evidence to the using of propolis.Propolis was added in dairy and the guarantee period was prolonged. More propolis makes longer guarantee period. Dairy was added 0.1% propolis after sterilized at 90℃for 5min , guarantee period was prolonged to 10 days. In other words, propolis was more effective than sodium benzoate as a chemical antistaling agent. Thus, propolis could be used as a nature substance, replace sodium benzoate, has a glorious future.