| As a product of modern biotechnology, glyphosate resistant soybean reduces people's labor intensity and weed control costs. For these reasons, its planting area has increased year by year where it has been allowed to plant. Although our country does not allow large-scale planting glyphosate-resistant soybeans at this stage, because china's increasing demand for soybeans, glyphosate resistant soybean plant will be allowed in the near future.Therefore, it is necessary to introduce and research before its arriving. Heilongjiang as one of the main soybean producing areas, it is more necessary to introduce and research climate adaptation of glyphosate resistant soybean after spraying glyphosate. On the one hand this research can play a role in the future introduction of glyphosate resistant soybean in Heilongjiang; on the other hand, it may be helpful to study the mechanism of glyphosate.In this study, we choose Dongnong 42 (one of the varieties grown in Heilongjiang province) and glyphosate resistant soybean for the material.First of all, we did molecular identification of glyphosate resistant soybean, to determine transferred genes.Then we sprayed glyphosate on soybean. We study physiology and stucture of them. The experimental results are as follows:1 Through the PCR of promoter, terminator, and cp4-epsps gene, it informed that glyphosate resistant soybean tranferred cp4-epsps gene.2 Through measuring chlorophyll, malondialdehyde, shikimic acid, protein and sugar content we have found that glyphosate on Dongnong 42's destruction were great. It made a devastating injury in a week: it destroyed chlorophyll; increased MDA ; declined soluble protein content; accumulated shikimic acid; increased soluble sugar and sucrose content; decreased starch content.Shikimic acid accumulated obviously in the lesves sprayed and the below one. Spraying of glyphosate as well as the adjacent pod and the pod loading adjacent Although there was shikimic acid accumulation in the sprayed pods, adjacent pods and the lealves above, it was not obvious.In addition, the accumulation of shikimic acid and the amount of glyphosate spraying had a great relationship.The more glyphosate we sprayed, the more shikimic acid accumulated. Besides,glyphosate sensitivity declined in period of podding. Shikimic acid accumulation also decreased in podding stage .After spraying glyphosate on glyphosate resistant soybean, MDA and shikimic acid content did not change apparently; Chlorophyll, protein, sugar and starch content, at first changed,but after several days they had recovery trends. The change of spraying 2 times was similar to spraying 1 time only.The result of 100 seed weight was no spraying>spraying 1 time >spraying 2 times.3 After spraying glyphosate, Dongnong 42 leaves gradually turned yellow; the cells broke down; the deformation of epidermal cells, stomata, glands increased; osmiophilic particles appeared, film layer structure became thiner, the starch grains decreased; chloroplast membrane was off the cell wall, cells collapsed.Glyphosate resistant soybean leaves had been maintaining the green; cells were in order; epidermal cells, stomata, glands, had a deformation after spraying glyphosate, and then gradually restored; Vacuole, chloroplast, chloroplast lamellae, starch grains changed, but then restore; Osmiophilic granules increased first then reduced, at last disappeared; there was a chloroplast vacuole after spraying glyphosate, but then disappeared.There were convexes near cells wall, but about a week later they disappeared.The change of trichomes glandular diameter, stomatal apparatus size, stomatalpore size, palisade tissue cells size, as well as leaf thickness, was a gradual restore after a deviation from the control. The change trend of spraying 2 times was similar to spraying 1 time only.
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