Research On The Levels And Speciations Of Eight Mineral Elements In Chinese Herbal Medicine Of Clearing Away Heat And Toxic Material

The contents,primary,secondary-level speciation of 8 mineral elements (Mg,Ca,Fe,Zn,Cu,Mn,Co﹑Cd),which contained in eight Chinese herbal medicines of clearing away heat and toxic Material for Bovine mastitis,was studied for the first time. The contents of 8 mineral elements in eight Chinese herbal medicines were determined by atomic absorption spectrometry , the relationship between the contentand their effect was discussed. The study show that the better digesting results were obtained with the use of 4:1(v/v)HNO3+HClO4. The content of Mg﹑Ca﹑Fe﹑Mn are abundant in eight Chinese herbal medicines. The content of harmful element (Cd) is very low. The average Zn/Cu ratio of seven Chinese herbal medicines is 16.88 that is higher than it in vivo of milch cow of Bovine mastitis. All these may be related to the effect of those eight Chinese herbal medicines for curing Bovine mastitis disease. The results offered some academic reference in selecting medicine and speciation analysis. Primary speciation of eight elements(Mg,Ca,Fe,Zn,Cu,Mn,Co﹑Cd) in eight Chinese herbal medicines were studied. The contents of sample of primary speciation obtained from primary analytical flow were determined by atomic absorption spectrometry,species distributions of the primary speciation analysis,such as extractive rate,immerse-residue ratio etc were also calculated. The concentration of eight element in medicines'residue were high,which showed that the residue were still valuable. That extractive rate of Mg﹑Ca﹑Fe and Mn was bigger,except for it different in each medicine. More datum and useful information was gained by traditional decoction. Therefore,the result of primary speciation offered some academic reference for using amount of Chinese herbal medicine,conforming analytical methods of higher speciation and exploring reasons of curing disease about Chinese herbal medicine.Secondary-level speciation of Mg﹑Ca﹑Fe and Mn in Fiveleaf Gynostemma Herb was studied on the base of the analysis of primary speciation. The results of secondary-level speciation were obtained by using cation-exchange resin,Amberlite XAD-2 macroreticular resin and Chelex-100 chelating resin. Four elements almost existed on free ions with little non free ions. Mg﹑Ca﹑Mn almost existed on inorganic forms,but Fe had half organic and inorganic. While Fe all existed on labile forms and Mg had almost,Ca and Mn both had stable and labile forms.