Isolation And Identification Of Porcine Reproductive And Respiratory Syndrome Virus And The Research Of The Immune Effect Of Inactivated Vaccine

Porcine reproductive and respiratory syndrome (PRRS) is a porcine reproductive and respiratory syndrome virus (PRRSV) cause obstacles and breeding sows in newborn piglets respiratory symptoms of a serious infectious disease against the pig farming industry. From the start by the end of May 2006, is located in South, East China region, Jiangxi and Hunan provinces, the growth of some farms and some sows bred Pig, Pig conservation rapid spread of an outbreak of a high mortality rate, cure rate and low variability in porcine reproductive and respiratory syndrome virus (NSP2 strains) as the leading factor causing the Pig "high fever" disease. According to reports, PRRSV genome a total of six kinds of structural protein genes, respectively, by the ORF2-7 gene encoding the virus GP2, GP3, GP4, GP5, M protein and N protein, ORF7 gene encoding the N protein is the main structural protein, for Identification of PRRSV with specificity.In this study, from Zhengzhou City, the incidence of a pig farm can be separated into a Marc-145 cells to produce virus diseases. According to porcine reproductive and respiratory syndrome virus (PRRSV) American-type standard strain ATCC VR-2332 has been published sequence (GenBank Accession No.PRU87392) designed to amplify the entire ORF7 gene fragments including a pair of specific primers, through its N protein gene identification analysis.Choose non-negative for PRRSV antibody and nucleic acid of the 30-day-old piglets, in accordance with the requirements of a porcine reproductive and respiratory syndrome vaccine immunization, the use of ELISA method to detect antibodies generated in order to know the situation after piglets immune antibody dynamics. Pig after the trial division at different stages of the use of immune ZZ attack isolates from drug testing, can find a protective antibody level of force. Number of different immune pigs during their growth in the antibody produced by the situation, in order to formulate scientific and rational basis for immunization procedures.The results show that RT-PCR method for identification of virus isolated, identified as PRRSV, strains of PRRSV ZZ named. ELISA detection of the antibody level reached 0.8 (S / P value) on the above with isolates of PRRSV have a certain degree of protection, and pigs showed only one had clinical symptoms; antibody levels to reach 1.5 (S / P value) above , be able to completely resist the immune Pig PRRSV, did not show any clinical symptoms. Against non-PRRSV antibody and nucleic acid-negative for a 30-day-old piglets after immunization, respectively, at different time periods and then a second immunization, for antibody detection, the results confirm that in the 60-day-old or 75-day-old secondary immunization can make pigs at slaughter in front of antibody levels are maintained at 0.8 and 1.5 (S / P value) between.This study has successfully separated a PRRSV, and of porcine reproductive and respiratory syndrome vaccine immune antibodies in piglets after the dynamics of the research, formulate scientific and rational for the porcine reproductive and respiratory syndrome vaccine immunization procedures and the Pig reproductive and respiratory syndrome prevention and control has made useful explorations.