Correlation Analysis Of Microsatellite DNA Markers With Some Substantial Economic Traits In High Quality Fine Wool Strain Of Gansu Alpine Fine-Wool Sheep

The genetic diversity of 153 samples in High Quality Fine Wool Strain of Gansu alpine Fine Wool sheep were studied,by using fifteen microsatellite loci.Microsatellite markers-phenotype correlation analysis was made by SPSS13.0.The results indicated that there had high genetic polymorphism at fourteen loci except one locus among the 15 microsatellite loci.The mean number of alleles was 10, effective number of alleles 7.1,mean heterozygosity 0.85,polymorphisms information content 0.83.It showed that High Quality Fine Wool Strain of Gansu Alpine Fine Wool Sheep had richer genetic diversity and more selection potential,so we can strengthen the process of selection and breeding.Correlation analysis of those 14 polymorphic Microsatellite loci with economic phenotypes showed that there were significant correlation(P＜0.05) or extreme significant correlation(p＜0.01) among 7 microsatellite loci with wool and weight traits. They are as following:BMS1724 has a significant impact on weaning staple length, BM6506 a extreme significant impact on weaning staple length;BL-4 and BMS1248 a significant impact on staple length at 12 months,BM6506 a extreme significant impact on staple length at 12 months;BM6506 a significant impact on belly staple length at 12 months;BL-4,BMS1248 and BMS 1724 a significant impact on greasy fleece weight at 12 months,BM6506 a extreme significant impact on greasy fleece weight at 12 months; BL-4 a significant impact on clean fleece weight at 12 months,BMS1724 a significant impact on clean fleece weight at 12 months and mean wool fibre diameter at 12 months, BM6506 a extreme significant impact on clean fleece weight at 12 months and mean wool fibre diameter at 12 months;URB037 a significant impact on clean wool yield at 12 months,MCM38 a extreme significant impact on clean wool yield;FCB48 and MCM38 a significant impact on coefficient of variation of wool fibre diameter at 12 months;BMS1248 and BM6506 have a significant impact on body weight at birth; BMS1248 and BMS 1724 have a significant impact on yearling weight.The multiple comparison was made between genotypes and the markers which have correlations with economic traits.The results are as following:Considering weaning staple length,186/186 is the dominant genotype in the marker BM6506; 156/168,160/176,164/176 and 164/186 are the dominant genotypes in the marker BMS1724.Considering staple length at 12 months,186/186 is the dominant genotype in the marker BM6506;140/160 and 142/160 are the dominant genotypes in the marker BMS1248;149/169 is the dominant genotype in the marker BL-4.Considering belly staple length at 12 months,186/186 is the dominant genotype in the marker BM6506. Considering greasy fleece weigh at 12 months,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;192/192,192/202 and 194/202 in the marker BM6506;149/169 is the dominant genotype in the marker BL-4; 160/176,164/176,164/186 and 174/198 are the dominant genotypes in the marker BMS1724.Considering clean fleece weight at 12 months,149/169,155/173,155/179 and 161/179 are the dominant genotypes in the marker BL-4;192/192,192/202 and 194/202 in the marker BM6506;160/176,164/176,164/186 and 174/198 in the marker BMS1724. Considering clean wool yield at 12 months,129/151,131/157,135/151 and 145/163 are the dominant genotypes in the marker MCM38;178/196,196/208,196/212 and 196/218 in the marker URB037.Considering mean wool fibre diameter at 12 months, 190/190,190/198 and 198/198 are the dominant genotypes in the marker BM6506; 178/204 is the dominant genotype in the marker BMS1724.Considering coefficient of variation of wool fibre diameter at 12 months,135/155,149/159 and 149/169 are the dominant genotypes in the marker FCB48;141/157 and 145/163 in the marker MCM38. Considering body weight at birth,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;186/186,188/188,192/192 and 192/202 in the marker BM6506. Considering yearling weight,140/160 and 142/160 are the dominant genotypes in the marker BMS1248;160/176,164/176,164/186,174/198 and 178/204 in the marker BMS1724;186/186,188/188,192/192 and 192/202 in the marker BM6506.