Genetic Diversity Analysis And Functional Gene Research Of Medicinal Plants Ginkgo Biloba L. And Paris L.

The research on genetic diversity is the premise and the foundation for analyzing and using medicinal plant resources,and is also studied for the origin and evolution of species.Only carries on a comprehensive and accurate understanding of the genetic diversity of a species we can take effective measures to protect and use.The medicinal ingredients in medicinal plants are products of their unique genes.Therefore,the most needed to protect is "functional genes related to the secondary metabolite synthesis".To search for expression law of functional genes related active ingredients of Chinese medicine and to determine the regulation mechanism of synthesis of medicinal ingredients is to lay the foundation for the modernization of traditional Chinese medicine.Using the RAPD-SCAR technology,to explore gender Ginkgo differences between the genome of the Ginkgo biloba for early identification of gender has important theoretical and practical value.This study obtained gender-related molecular markers of Ginkgo biloba,and applied to samples of unknown sex of Ginkgo biloba.The molecular markers can accurately identify the sex of Ginkgo biloba.Paris L.has great variation in the form between different groups, and the speed of evolution of morphological traits is not synchronized,so the difficulties of classification increase.Therefore,the study of genetic differentiation of Paris has a great significance for the system classification in Paris.In our study,matK gene,trnL_F,ITS sequences are used as molecular targets.The use of RAPD molecular markers is to explore the genetic diversity and genetic differentiation of Paris.Analysis of these two methods was approximately the same results,and its classification and genetic relationship between species were described.Research on steroidal saponins of Paris in the biosynthetic pathway of functional genes has an important practical significance for us to understand the active ingredients of medicinal plants and the impact of factors.By using Real-time PCR,we can detect the two functional differences in gene expression in three-part organization of two kinds of Paris.The experiments showed that genes HMGCoA reductase and squalene epoxidase in the root and stem are more highly expressed than leaves,especially in the highest expression of the root.This was accorded with the steroidal saponins of Paris was mainly existed in the root and stem,and roots have greater medicinal value than leaves.It also proved that the genes HMGCoA reductase and squalene epoxidase are assuredly related to the biosynthetic pathway of steroidal saponins.The expression level of gene HMGCoA reductase and squalene epoxidase affected the biosynthesis of steroidal saponin.Because these two functional genes have been little studied,and the regulation mechanism of steroidal saponins biosynthetic pathway is very complex,it needs further research.