Study On Sperm Separation And Sex Control Technology In Red Deer

Sperm separation and artificial insemination of livestock are the most available gender control technologies.The experiments studied the transportation of the red deer fresh semen,the conditions of X/Y sperm sorting,and the possibility of production of sexed Red deer by AI apply sexed semen.The results were showing as following:①The results showed that the transportion temperature control of ejaculated semen was very important.After the addition of CSS antibiotics,which the ejaculated semen were incubated at 18℃water was most appropriate.②The results showed that the sorting speed of among three samples were significant difference(P＜0.05) depending on the rate of fresh sperm motility.③the motility(0.61 vs 0.60 vs 0.65)and 4 hour motility(0.30 vs 0.30 vs0.32) of sorted frozed sperm had no significant differences(P＞0.05 ) which produced from three ejaculations of same donor.④80 natural estrous Red deers were inseminated by uterine horn AI with 2×106 Y sorted froze-thawed spermatozoa.The rates of pregnant and sex accuracy was 67.5%and 100%,repectively.In general,Those results indicate that the technologies of X/Y sperm sorting and the production of sexed red deer by using deep-AI will become an useful technologies in Red deer sex selected reproduction in the future.