Studies On Plant Regeneration System Of Cherry In Different Genotypes

As In Vitro leaf,leafstalk,internodes and root the material ,the study is to optimize and open up tissue culuture and rapid propagation system in different gene cherry,and discuss regeneration system of cherry genetic transformation.In Vitro construction of regeneration system for different selections of cherry plant hybrid including'F8'(Prunus cerasus L×Prunus.pseudocerasus L.),'F10'(Prunus. avium L.×Prunus. pseudocerasus L.),'H8'(Prunus. cerasus L×Prunus. pseudocerasus L.and'H10'(Prunus cerasus L×Prunus pseudocerasus L.) were studied.It was showed that by taking MS and F14 basic medium supplemented with different combinations of plant growth regulators such as IBA and GA3,the micropropagation technologies including preliminary culture, subculture and rooting culture were successfully established.Medium F14+6-BA(0.3~0.5)mg/L+GA3 0.1mg/L is suitable for preliminary culture of the tested selections,F14+6-BA(0.5~1.0)mg/L+IBA0.1mg/L+ GA30.1mg/L is an optimal subculture medium for proliferation of the In Vitro shoots, satisfact ory rooting response can be obtained on medium 1/2 MS+IBA 0.5 mg/L(or NAA0.8 mg/L).The patterns of plant regeneration in different genotypes of cherry plants,including sweet cherry cultivars'meizao'and'Summit',hybrid lines'F8','F10','H8'and'H10'were comparatively studied, especially focused on the regeneration technology of the several hybrid lines.It was showed that cherry plants with different genetic background take specifically patterns and routes for plant regeneration. Satisfactory regenerations can be obtained in different materials by chose In Vitro leaves, leafstalks, internodes and root systems as explants,and by taking special induction and culture manipulations, including the basic mediums, levels and combinations of plant growth regulators.The primary results is as follows:above 90.0% of In Vitro leaves of sweet cherry cultivar'Meizao'regenerate adventitious buds on WPM+6-BA2.0 mg/L+IAA2.0mg/L+GA30.1mg/L',H10'',Summit'',F10','F8'can also get 76.0%,86.0%, 80.0%,64.0% regeneration respectively in 6-BA(2~2.5mg/L) and IAA(1.0~2.0mg/L) In addition, choosing young leaves at upper part of the tube plantlet and culture in darkness for 1 week, with the axial surface contact with the medium benefit to adventitious bud regeneration.when taken In Vitro leafstalks of hybrid'H10', 40.0% regeneration can be obtained on modified MS+6-BA2.0 mg/L+IBA0.1mg/L +2,4-D0.5 mg/L,only'H8'lesfstalk get a small quantity of regeneration plant in others. In addition, leafstalk with a piece of leaf section and treatment with 5mg/L of AgNO3 improved adventitious shoot regeneration In Vitro leafstalk explants.while the medium LS+6-BA 1.0 mg/L+NAA2.0 mg/L+CH100 mg/L is optimal for regeneration of hybrid'F10',40.0% regeneration can be obtained, etiolation treatment internodes gave less adventitious shoots than that of un-treated ones.As high as 96.7% of intact root system of hybrid H8 regenerate buds on MS+6-BA1.0 mg/L +2,4-D2.0 mg/L +IBA0.05mg/L+NAA0.05 mg/L. H8,H10 are 25.0% and 30.0% in intro internode regeneration respectively, while'H10'',F8'',F10'are 93.3%,96.7%, 93.3% in intro root regeneration respectively in other concentration. More adventitious shoots regeneration can be obtained on intact root system than that of root sections.Adventitious root regeneration was also tested by taking In Vitro internodes of'H8'and leaves of'duiying'as materials. It was showed that on MS basic medium supplement with NAA1.0 mg/L,64.0% and 80.0% root regeneration can be obtained respectively, concentration of NAA, higher or lower than 1.0 mg/L decreased root induction. The result indicated that plant regeneration can be indirectly achieved via adventitious root regarding to the materials in which direct adventitious bud regeneration is not easy to be obtained.