Development Of Inactivated Vaccines And Diagnostic Methods Of Equine Influenza Virus Subtype H3N8

Equine influenza shorter for EIV, is that the reason is gluing virus section the (Orthomyxoviridae) influenza virus belongs to the (Influenza virus) horse A type influenza virus (EIV) arouses one kind of dyadic popular impatient outbreak infectious disease of Equine animal.It is one of the most serious infectious diseases and impacted horse industry and horse racing industry. World Organization for Animal Health as a statutory report of its animal disease. In October 2007, located in the northern part of China's Xinjiang Autonomous Region Fuyun County Horse flu suspected cases has been found, and spread rapidly from west to east. Researchers from our lab is expected to disease acquisition, and successfully isolated horse influenza virus. After identification, Determined as the H3N8 subtype of EIV. During 2008, many strains of virus isolated from Liaoning, Inner Mongolia, Wuhan, etc. Because of the horse flu outbreak happened at the eve of the Beijing Olympic Games, It threated the match race of horse for the Olympic Games seriously. A convenient and accurate diagnosis of equine influenza is urgently needed for horse during the pathogenic influenza pandemic. This study set up two methods about Serological, one is Hemagglutination-hemagglutination inhibition test, the other is indirect ELISA method, and successfully applied to the practice of testing. The establishment of serological diagnostic methods is sensitivity, good reproducibility, specificity, etc. giving a good reference to Horse flu diagnostic reagents for the development of the foundation.At present, the development of our country horse influenza inactivated vaccine have three kinds belong H7N7 type, H3N8 type, and Bivalent vaccine, but no large-scale application. Once the horse flu has occurred, the main measures is taken by some symptomatic treatment. Horse flu virus like other influenza viruses, it is variation Frequently.Accompanied by Horse industry and horse racing industry Development ,the Increase in the number of International horse mobile, It is imperative to develop new vaccines. In this study, the prevalence of domestic horse flu strain as a drug A/equine/xinjiang/03/07,by two different adjuvants and diffrent hemagglutination titer methods subtypeH3N8 inactivated vaccine of equine influenza has been development.Through the security experiments, the effectiveness of the experiment,we found the best conditions for high-level of vaccine-induced antibodies and provide a reference for Subtype H3N8 inactivated vaccine.