Effects Of Copper And Manganese On Semen Quality And Antioxygenic Property In Stud Holstein Bulls

This paper studied the effects of different levels of Copper and Manganese on the semen quality, antioxygenic property,and blood biochemical parameters of stud Holstein bulls. Expt.1 Forty breeding Holstein bulls,which all aspects were not significant,were randomly divided into ten groups.The control group was fed with basic diet the others were fed with three levels of copper 4, 8,12 mg/kg(on the basis of dry matter) and three levels of manganese 50,125,200 mg/kg(on the basis of dry matter) in the basal diet Experimental design was used by 3×3 trial design.The experiment lasted 8 months.The results showed that the sperm motility of the experimental group was higher than the contol group significant(P<0.05),and the abnormalities were lower than the control group significantly (P<0.05).The ejaculation,freeze-thawed motility and concentration were better than the control group,but had no significant difference(P>0.05).It was concluded that basal diet supplementing with 4 mg/kg copper and 125 mg/kg manganese were the optimal supplementation in this experiment according to analysis of semen quality parameters.Expt.2 Forty breeding Holstein bulls,which all aspects were not significant,were randomly divided into ten groups.The control group was fed with basic diet the others were fed with three levels of copper 4, 8,12 mg/kg(on the basis of dry matter) and three levels of manganese 50,125,200 mg/kg(on the basis of dry matter) in the basal diet Experimental design was used by 3×3 trial design.The experiment lasted 8 months.The results indicated that the activities of GSH-Px,SOD,Mn-SOD and the content of NO was the extremely significant(P<0.01) when basic diet was added different levels of copper and manganese,the significant difference of the activities of T-AOC and CuZn-SOD(P<0.05),and not significant difference of the activities of CP,CAT and the content of MDA(P>0.05).It was concluded that basal diet supplementing with 4～8 mg/kg copper and 50 mg/kg manganese were the optimal supplementation in this experiment according to analysis of all blood antioxidation parameters.